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Morphological effect of oscillating magnetic nanoparticles in killing tumor cells

Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNP...

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Detalles Bibliográficos
Autores principales: Cheng, Dengfeng, Li, Xiao, Zhang, Guoxin, Shi, Hongcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019356/
https://www.ncbi.nlm.nih.gov/pubmed/24872797
http://dx.doi.org/10.1186/1556-276X-9-195
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author Cheng, Dengfeng
Li, Xiao
Zhang, Guoxin
Shi, Hongcheng
author_facet Cheng, Dengfeng
Li, Xiao
Zhang, Guoxin
Shi, Hongcheng
author_sort Cheng, Dengfeng
collection PubMed
description Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNPs mechanically damaged cell membranes and cytoplasm, decreasing the cell viability. It was found that the concentration and morphology of the MNPs significantly influenced the cell-killing efficiency of oscillating MNPs. In this preliminary study, when HeLa cells were pre-incubated with 100 μg/mL rod-shaped MNPs (rMNP, length of 200 ± 50 nm and diameter of 50 to 120 nm) for 20 h, MTT assay proved that the cell viability decreased by 30.9% after being exposed to AMF for 2 h, while the cell viability decreased by 11.7% if spherical MNPs (sMNP, diameter of 200 ± 50 nm) were used for investigation. Furthermore, the morphological effect of MNPs on cell viability was confirmed by trypan blue assay: 39.5% rMNP-loaded cells and 15.1% sMNP-loaded cells were stained after being exposed to AMF for 2 h. It was also interesting to find that killing tumor cells at either higher (500 μg/mL) or lower (20 μg/mL) concentration of MNPs was less efficient than that achieved at 100 μg/mL concentration. In conclusion, the relatively asymmetric morphological rod-shaped MNPs can kill cancer cells more effectively than spherical MNPs when being exposed to AMF by virtue of their mechanical oscillations.
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spelling pubmed-40193562014-05-28 Morphological effect of oscillating magnetic nanoparticles in killing tumor cells Cheng, Dengfeng Li, Xiao Zhang, Guoxin Shi, Hongcheng Nanoscale Res Lett Nano Express Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNPs mechanically damaged cell membranes and cytoplasm, decreasing the cell viability. It was found that the concentration and morphology of the MNPs significantly influenced the cell-killing efficiency of oscillating MNPs. In this preliminary study, when HeLa cells were pre-incubated with 100 μg/mL rod-shaped MNPs (rMNP, length of 200 ± 50 nm and diameter of 50 to 120 nm) for 20 h, MTT assay proved that the cell viability decreased by 30.9% after being exposed to AMF for 2 h, while the cell viability decreased by 11.7% if spherical MNPs (sMNP, diameter of 200 ± 50 nm) were used for investigation. Furthermore, the morphological effect of MNPs on cell viability was confirmed by trypan blue assay: 39.5% rMNP-loaded cells and 15.1% sMNP-loaded cells were stained after being exposed to AMF for 2 h. It was also interesting to find that killing tumor cells at either higher (500 μg/mL) or lower (20 μg/mL) concentration of MNPs was less efficient than that achieved at 100 μg/mL concentration. In conclusion, the relatively asymmetric morphological rod-shaped MNPs can kill cancer cells more effectively than spherical MNPs when being exposed to AMF by virtue of their mechanical oscillations. Springer 2014-04-28 /pmc/articles/PMC4019356/ /pubmed/24872797 http://dx.doi.org/10.1186/1556-276X-9-195 Text en Copyright © 2014 Cheng et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Nano Express
Cheng, Dengfeng
Li, Xiao
Zhang, Guoxin
Shi, Hongcheng
Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title_full Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title_fullStr Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title_full_unstemmed Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title_short Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
title_sort morphological effect of oscillating magnetic nanoparticles in killing tumor cells
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019356/
https://www.ncbi.nlm.nih.gov/pubmed/24872797
http://dx.doi.org/10.1186/1556-276X-9-195
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AT lixiao morphologicaleffectofoscillatingmagneticnanoparticlesinkillingtumorcells
AT zhangguoxin morphologicaleffectofoscillatingmagneticnanoparticlesinkillingtumorcells
AT shihongcheng morphologicaleffectofoscillatingmagneticnanoparticlesinkillingtumorcells