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Morphological effect of oscillating magnetic nanoparticles in killing tumor cells
Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNP...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019356/ https://www.ncbi.nlm.nih.gov/pubmed/24872797 http://dx.doi.org/10.1186/1556-276X-9-195 |
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author | Cheng, Dengfeng Li, Xiao Zhang, Guoxin Shi, Hongcheng |
author_facet | Cheng, Dengfeng Li, Xiao Zhang, Guoxin Shi, Hongcheng |
author_sort | Cheng, Dengfeng |
collection | PubMed |
description | Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNPs mechanically damaged cell membranes and cytoplasm, decreasing the cell viability. It was found that the concentration and morphology of the MNPs significantly influenced the cell-killing efficiency of oscillating MNPs. In this preliminary study, when HeLa cells were pre-incubated with 100 μg/mL rod-shaped MNPs (rMNP, length of 200 ± 50 nm and diameter of 50 to 120 nm) for 20 h, MTT assay proved that the cell viability decreased by 30.9% after being exposed to AMF for 2 h, while the cell viability decreased by 11.7% if spherical MNPs (sMNP, diameter of 200 ± 50 nm) were used for investigation. Furthermore, the morphological effect of MNPs on cell viability was confirmed by trypan blue assay: 39.5% rMNP-loaded cells and 15.1% sMNP-loaded cells were stained after being exposed to AMF for 2 h. It was also interesting to find that killing tumor cells at either higher (500 μg/mL) or lower (20 μg/mL) concentration of MNPs was less efficient than that achieved at 100 μg/mL concentration. In conclusion, the relatively asymmetric morphological rod-shaped MNPs can kill cancer cells more effectively than spherical MNPs when being exposed to AMF by virtue of their mechanical oscillations. |
format | Online Article Text |
id | pubmed-4019356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-40193562014-05-28 Morphological effect of oscillating magnetic nanoparticles in killing tumor cells Cheng, Dengfeng Li, Xiao Zhang, Guoxin Shi, Hongcheng Nanoscale Res Lett Nano Express Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNPs mechanically damaged cell membranes and cytoplasm, decreasing the cell viability. It was found that the concentration and morphology of the MNPs significantly influenced the cell-killing efficiency of oscillating MNPs. In this preliminary study, when HeLa cells were pre-incubated with 100 μg/mL rod-shaped MNPs (rMNP, length of 200 ± 50 nm and diameter of 50 to 120 nm) for 20 h, MTT assay proved that the cell viability decreased by 30.9% after being exposed to AMF for 2 h, while the cell viability decreased by 11.7% if spherical MNPs (sMNP, diameter of 200 ± 50 nm) were used for investigation. Furthermore, the morphological effect of MNPs on cell viability was confirmed by trypan blue assay: 39.5% rMNP-loaded cells and 15.1% sMNP-loaded cells were stained after being exposed to AMF for 2 h. It was also interesting to find that killing tumor cells at either higher (500 μg/mL) or lower (20 μg/mL) concentration of MNPs was less efficient than that achieved at 100 μg/mL concentration. In conclusion, the relatively asymmetric morphological rod-shaped MNPs can kill cancer cells more effectively than spherical MNPs when being exposed to AMF by virtue of their mechanical oscillations. Springer 2014-04-28 /pmc/articles/PMC4019356/ /pubmed/24872797 http://dx.doi.org/10.1186/1556-276X-9-195 Text en Copyright © 2014 Cheng et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Nano Express Cheng, Dengfeng Li, Xiao Zhang, Guoxin Shi, Hongcheng Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title | Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title_full | Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title_fullStr | Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title_full_unstemmed | Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title_short | Morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
title_sort | morphological effect of oscillating magnetic nanoparticles in killing tumor cells |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019356/ https://www.ncbi.nlm.nih.gov/pubmed/24872797 http://dx.doi.org/10.1186/1556-276X-9-195 |
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