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Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019481/ https://www.ncbi.nlm.nih.gov/pubmed/24823362 http://dx.doi.org/10.1371/journal.pone.0096288 |
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author | Srinivasan, Vijaya Bharathi Singh, Bharat Bhushan Priyadarshi, Nitesh Chauhan, Neeraj Kumar Rajamohan, Govindan |
author_facet | Srinivasan, Vijaya Bharathi Singh, Bharat Bhushan Priyadarshi, Nitesh Chauhan, Neeraj Kumar Rajamohan, Govindan |
author_sort | Srinivasan, Vijaya Bharathi |
collection | PubMed |
description | BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae. |
format | Online Article Text |
id | pubmed-4019481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40194812014-05-16 Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae Srinivasan, Vijaya Bharathi Singh, Bharat Bhushan Priyadarshi, Nitesh Chauhan, Neeraj Kumar Rajamohan, Govindan PLoS One Research Article BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae. Public Library of Science 2014-05-13 /pmc/articles/PMC4019481/ /pubmed/24823362 http://dx.doi.org/10.1371/journal.pone.0096288 Text en © 2014 Srinivasan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Srinivasan, Vijaya Bharathi Singh, Bharat Bhushan Priyadarshi, Nitesh Chauhan, Neeraj Kumar Rajamohan, Govindan Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae |
title | Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
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title_full | Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
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title_fullStr | Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
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title_full_unstemmed | Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
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title_short | Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
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title_sort | role of novel multidrug efflux pump involved in drug resistance in klebsiella pneumoniae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019481/ https://www.ncbi.nlm.nih.gov/pubmed/24823362 http://dx.doi.org/10.1371/journal.pone.0096288 |
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