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Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae

BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. p...

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Autores principales: Srinivasan, Vijaya Bharathi, Singh, Bharat Bhushan, Priyadarshi, Nitesh, Chauhan, Neeraj Kumar, Rajamohan, Govindan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019481/
https://www.ncbi.nlm.nih.gov/pubmed/24823362
http://dx.doi.org/10.1371/journal.pone.0096288
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author Srinivasan, Vijaya Bharathi
Singh, Bharat Bhushan
Priyadarshi, Nitesh
Chauhan, Neeraj Kumar
Rajamohan, Govindan
author_facet Srinivasan, Vijaya Bharathi
Singh, Bharat Bhushan
Priyadarshi, Nitesh
Chauhan, Neeraj Kumar
Rajamohan, Govindan
author_sort Srinivasan, Vijaya Bharathi
collection PubMed
description BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae.
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spelling pubmed-40194812014-05-16 Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae Srinivasan, Vijaya Bharathi Singh, Bharat Bhushan Priyadarshi, Nitesh Chauhan, Neeraj Kumar Rajamohan, Govindan PLoS One Research Article BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae. Public Library of Science 2014-05-13 /pmc/articles/PMC4019481/ /pubmed/24823362 http://dx.doi.org/10.1371/journal.pone.0096288 Text en © 2014 Srinivasan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Srinivasan, Vijaya Bharathi
Singh, Bharat Bhushan
Priyadarshi, Nitesh
Chauhan, Neeraj Kumar
Rajamohan, Govindan
Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title_full Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title_fullStr Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title_full_unstemmed Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title_short Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae
title_sort role of novel multidrug efflux pump involved in drug resistance in klebsiella pneumoniae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019481/
https://www.ncbi.nlm.nih.gov/pubmed/24823362
http://dx.doi.org/10.1371/journal.pone.0096288
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