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Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes
Septins are filament-forming proteins important for organizing the cortex of animal and fungal cells. In mammals, 13 septin paralogues were recently shown to assemble into core heterohexamer and heterooctamer complexes, which serve as building blocks for apolar filamentous structures that differ amo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019491/ https://www.ncbi.nlm.nih.gov/pubmed/24648497 http://dx.doi.org/10.1091/mbc.E13-09-0553 |
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author | Sellin, Mikael E. Stenmark, Sonja Gullberg, Martin |
author_facet | Sellin, Mikael E. Stenmark, Sonja Gullberg, Martin |
author_sort | Sellin, Mikael E. |
collection | PubMed |
description | Septins are filament-forming proteins important for organizing the cortex of animal and fungal cells. In mammals, 13 septin paralogues were recently shown to assemble into core heterohexamer and heterooctamer complexes, which serve as building blocks for apolar filamentous structures that differ among cell types. To determine how tissue-specific septin paralogue expression may shape core heteromer repertoires and thereby modulate properties of septin filaments, we devised protocols to analyze native septin heteromers with distinct numbers of subunits. Our evidence based on genetically manipulated human cells supports and extends recent concepts of homology subgroup–restricted assembly into distinct categories of apolar heterohexamers and heterooctamers. We also identify a category of tetramers that have a subunit composition equivalent to an octameric building block. These atypical tetramers are prevalent in lymphocytes and neural tissues, in which octamers are abundant but hexamers are rare. Our results can be explained by tissue-specific expression of SEPT3 subgroup members: SEPT3, SEPT9, and SEPT12. These serve as cognate subunits in either heterooctamers or atypical tetramers but exhibit different preferences in various tissues. The identified tissue-specific repertoires of septin heteromers provide insights into how higher-order septin structures with differential properties and stabilities may form in diverse animal cell types. |
format | Online Article Text |
id | pubmed-4019491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-40194912014-07-30 Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes Sellin, Mikael E. Stenmark, Sonja Gullberg, Martin Mol Biol Cell Articles Septins are filament-forming proteins important for organizing the cortex of animal and fungal cells. In mammals, 13 septin paralogues were recently shown to assemble into core heterohexamer and heterooctamer complexes, which serve as building blocks for apolar filamentous structures that differ among cell types. To determine how tissue-specific septin paralogue expression may shape core heteromer repertoires and thereby modulate properties of septin filaments, we devised protocols to analyze native septin heteromers with distinct numbers of subunits. Our evidence based on genetically manipulated human cells supports and extends recent concepts of homology subgroup–restricted assembly into distinct categories of apolar heterohexamers and heterooctamers. We also identify a category of tetramers that have a subunit composition equivalent to an octameric building block. These atypical tetramers are prevalent in lymphocytes and neural tissues, in which octamers are abundant but hexamers are rare. Our results can be explained by tissue-specific expression of SEPT3 subgroup members: SEPT3, SEPT9, and SEPT12. These serve as cognate subunits in either heterooctamers or atypical tetramers but exhibit different preferences in various tissues. The identified tissue-specific repertoires of septin heteromers provide insights into how higher-order septin structures with differential properties and stabilities may form in diverse animal cell types. The American Society for Cell Biology 2014-05-15 /pmc/articles/PMC4019491/ /pubmed/24648497 http://dx.doi.org/10.1091/mbc.E13-09-0553 Text en © 2014 Sellin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Sellin, Mikael E. Stenmark, Sonja Gullberg, Martin Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title | Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title_full | Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title_fullStr | Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title_full_unstemmed | Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title_short | Cell type–specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes |
title_sort | cell type–specific expression of sept3-homology subgroup members controls the subunit number of heteromeric septin complexes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019491/ https://www.ncbi.nlm.nih.gov/pubmed/24648497 http://dx.doi.org/10.1091/mbc.E13-09-0553 |
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