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Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells

Angiogenesis is associated with the tissue changes underlying chronic overuse tendinopathy. We hypothesized that repetitive, cyclic loading of human tendon cells would lead to increased expression and activity of angiogenic factors. We subjected isolated human tendon cells to overuse tensile loading...

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Autores principales: Mousavizadeh, Rouhollah, Khosravi, Shahram, Behzad, Hayedeh, McCormack, Robert G., Duronio, Vincent, Scott, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019633/
https://www.ncbi.nlm.nih.gov/pubmed/24824595
http://dx.doi.org/10.1371/journal.pone.0097356
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author Mousavizadeh, Rouhollah
Khosravi, Shahram
Behzad, Hayedeh
McCormack, Robert G.
Duronio, Vincent
Scott, Alex
author_facet Mousavizadeh, Rouhollah
Khosravi, Shahram
Behzad, Hayedeh
McCormack, Robert G.
Duronio, Vincent
Scott, Alex
author_sort Mousavizadeh, Rouhollah
collection PubMed
description Angiogenesis is associated with the tissue changes underlying chronic overuse tendinopathy. We hypothesized that repetitive, cyclic loading of human tendon cells would lead to increased expression and activity of angiogenic factors. We subjected isolated human tendon cells to overuse tensile loading using an in vitro model (1 Hz, 10% equibiaxial strain). We found that mechanically stimulated human tendon cells released factors that promoted in vitro proliferation and tube formation by human umbilical vein endothelial cells (HUVEC). In response to cyclic strain, there was a transient increase in the expression of several angiogenic genes including ANGPTL4, FGF-2, COX-2, SPHK1, TGF-alpha, VEGF-A and VEGF-C, with no change in anti-angiogenic genes (BAI1, SERPINF1, THBS1 and 2, TIMP1-3). Cyclic strain also resulted in the extracellular release of ANGPTL4 protein by tendon cells. Our study is the first report demonstrating the induction of ANGPTL4 mRNA and release of ANGPTL4 protein in response to cyclic strain. Tenocytes may contribute to the upregulation of angiogenesis during the development of overuse tendinopathy.
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spelling pubmed-40196332014-05-16 Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells Mousavizadeh, Rouhollah Khosravi, Shahram Behzad, Hayedeh McCormack, Robert G. Duronio, Vincent Scott, Alex PLoS One Research Article Angiogenesis is associated with the tissue changes underlying chronic overuse tendinopathy. We hypothesized that repetitive, cyclic loading of human tendon cells would lead to increased expression and activity of angiogenic factors. We subjected isolated human tendon cells to overuse tensile loading using an in vitro model (1 Hz, 10% equibiaxial strain). We found that mechanically stimulated human tendon cells released factors that promoted in vitro proliferation and tube formation by human umbilical vein endothelial cells (HUVEC). In response to cyclic strain, there was a transient increase in the expression of several angiogenic genes including ANGPTL4, FGF-2, COX-2, SPHK1, TGF-alpha, VEGF-A and VEGF-C, with no change in anti-angiogenic genes (BAI1, SERPINF1, THBS1 and 2, TIMP1-3). Cyclic strain also resulted in the extracellular release of ANGPTL4 protein by tendon cells. Our study is the first report demonstrating the induction of ANGPTL4 mRNA and release of ANGPTL4 protein in response to cyclic strain. Tenocytes may contribute to the upregulation of angiogenesis during the development of overuse tendinopathy. Public Library of Science 2014-05-13 /pmc/articles/PMC4019633/ /pubmed/24824595 http://dx.doi.org/10.1371/journal.pone.0097356 Text en © 2014 Mousavizadeh et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mousavizadeh, Rouhollah
Khosravi, Shahram
Behzad, Hayedeh
McCormack, Robert G.
Duronio, Vincent
Scott, Alex
Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title_full Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title_fullStr Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title_full_unstemmed Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title_short Cyclic Strain Alters the Expression and Release of Angiogenic Factors by Human Tendon Cells
title_sort cyclic strain alters the expression and release of angiogenic factors by human tendon cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019633/
https://www.ncbi.nlm.nih.gov/pubmed/24824595
http://dx.doi.org/10.1371/journal.pone.0097356
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