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Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy

The therapeutic action of bone marrow-derived mesenchymal stem cells (BMSCs) in acute kidney injury (AKI) has been reported by several groups. However, recent studies indicated that BMSCs homed to kidney tissues at very low levels after transplantation. The lack of specific homing of exogenously inf...

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Autores principales: Liu, Pengfei, Feng, Yetong, Dong, Chao, Yang, Dandan, Li, Bo, Chen, Xin, Zhang, Zhongjun, Wang, Yi, Zhou, Yulai, Zhao, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019657/
https://www.ncbi.nlm.nih.gov/pubmed/24824427
http://dx.doi.org/10.1371/journal.pone.0097123
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author Liu, Pengfei
Feng, Yetong
Dong, Chao
Yang, Dandan
Li, Bo
Chen, Xin
Zhang, Zhongjun
Wang, Yi
Zhou, Yulai
Zhao, Lei
author_facet Liu, Pengfei
Feng, Yetong
Dong, Chao
Yang, Dandan
Li, Bo
Chen, Xin
Zhang, Zhongjun
Wang, Yi
Zhou, Yulai
Zhao, Lei
author_sort Liu, Pengfei
collection PubMed
description The therapeutic action of bone marrow-derived mesenchymal stem cells (BMSCs) in acute kidney injury (AKI) has been reported by several groups. However, recent studies indicated that BMSCs homed to kidney tissues at very low levels after transplantation. The lack of specific homing of exogenously infused cells limited the effective implementation of BMSC-based therapies. In this study, we provided evidence that the administration of BMSCs combined with muscone in rats with gentamicin-induced AKI intravenously, was a feasible strategy to drive BMSCs to damaged tissues and improve the BMSC-based therapeutic effect. The effect of muscone on BMSC bioactivity was analyzed in vitro and in vivo. The results indicated that muscone could promote BMSC migration and proliferation. Some secretory capacity of BMSC still could be improved in some degree. The BMSC-based therapeutic action was ameliorated by promoting the recovery of biochemical variables in urine or blood, as well as the inhibition of cell apoptosis and inflammation. In addition, the up-regulation of CXCR4 and CXCR7 expression in BMSCs could be the possible mechanism of muscone amelioration. Thus, our study indicated that enhancement of BMSCs bioactivities with muscone could increase the BMSC therapeutic potential and further developed a new therapeutic strategy for the treatment of AKI.
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spelling pubmed-40196572014-05-16 Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy Liu, Pengfei Feng, Yetong Dong, Chao Yang, Dandan Li, Bo Chen, Xin Zhang, Zhongjun Wang, Yi Zhou, Yulai Zhao, Lei PLoS One Research Article The therapeutic action of bone marrow-derived mesenchymal stem cells (BMSCs) in acute kidney injury (AKI) has been reported by several groups. However, recent studies indicated that BMSCs homed to kidney tissues at very low levels after transplantation. The lack of specific homing of exogenously infused cells limited the effective implementation of BMSC-based therapies. In this study, we provided evidence that the administration of BMSCs combined with muscone in rats with gentamicin-induced AKI intravenously, was a feasible strategy to drive BMSCs to damaged tissues and improve the BMSC-based therapeutic effect. The effect of muscone on BMSC bioactivity was analyzed in vitro and in vivo. The results indicated that muscone could promote BMSC migration and proliferation. Some secretory capacity of BMSC still could be improved in some degree. The BMSC-based therapeutic action was ameliorated by promoting the recovery of biochemical variables in urine or blood, as well as the inhibition of cell apoptosis and inflammation. In addition, the up-regulation of CXCR4 and CXCR7 expression in BMSCs could be the possible mechanism of muscone amelioration. Thus, our study indicated that enhancement of BMSCs bioactivities with muscone could increase the BMSC therapeutic potential and further developed a new therapeutic strategy for the treatment of AKI. Public Library of Science 2014-05-13 /pmc/articles/PMC4019657/ /pubmed/24824427 http://dx.doi.org/10.1371/journal.pone.0097123 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Pengfei
Feng, Yetong
Dong, Chao
Yang, Dandan
Li, Bo
Chen, Xin
Zhang, Zhongjun
Wang, Yi
Zhou, Yulai
Zhao, Lei
Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title_full Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title_fullStr Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title_full_unstemmed Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title_short Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
title_sort administration of bmscs with muscone in rats with gentamicin-induced aki improves their therapeutic efficacy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019657/
https://www.ncbi.nlm.nih.gov/pubmed/24824427
http://dx.doi.org/10.1371/journal.pone.0097123
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