Cargando…

Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s

BACKGROUND: The increasing status of insecticide resistant mosquitoes in sub-Saharan Africa is a threatening alert to the existing control efforts. All sibling species of An. gambiae complex have evolved insecticide resistance in wild populations for different approved classes of the insecticides cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Mdoe, France P, Cheng, Sen-Sung, Msangi, Shandala, Nkwengulila, Gamba, Chang, Shang-Tzen, Kweka, Eliningaya J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019784/
https://www.ncbi.nlm.nih.gov/pubmed/24885613
http://dx.doi.org/10.1186/1756-3305-7-209
_version_ 1782480214126755840
author Mdoe, France P
Cheng, Sen-Sung
Msangi, Shandala
Nkwengulila, Gamba
Chang, Shang-Tzen
Kweka, Eliningaya J
author_facet Mdoe, France P
Cheng, Sen-Sung
Msangi, Shandala
Nkwengulila, Gamba
Chang, Shang-Tzen
Kweka, Eliningaya J
author_sort Mdoe, France P
collection PubMed
description BACKGROUND: The increasing status of insecticide resistant mosquitoes in sub-Saharan Africa is a threatening alert to the existing control efforts. All sibling species of An. gambiae complex have evolved insecticide resistance in wild populations for different approved classes of the insecticides currently in use in the field. An alternative compound for vector control is absolutely urgently needed. In this study, the larvicidal activity and chemical composition of the Cinnamomum osmophloeum leaf essential oils were investigated. METHODS: C. osmophloeum leaf essential oils were extracted by hydrodistillation in a Clevenger-type apparatus for 6 hours, and their chemical compositions identified using GC-MS. These oils were evaluated against An. gambiae s.s. in both laboratory and semi-field situations. The WHO test procedures for monitoring larvicidal efficacy in malaria vectors were used. RESULTS: The composition of C. osmophloeum leaf essential oil has been found to have 11 active compounds. The most abundant compound was trans-cinnamaldehyde (70.20%) and the least abundant was caryophyllene oxide (0.08%). The larvicidal activity was found to be dosage and time dependant both in laboratory and semi-field environments with mortality ranging from 0% to 100%. The LC(50) value was found to vary from 22.18 to 58.15 μg/ml in the laboratory while in semi-field environments it was 11.91 to 63.63 μg/ml. The LC(90) value was found to range between 57.71 to 91.54 μg/ml in the laboratory while in semi-field environments was 52.07 to 173.77 μg/ml. Mortality ranged from 13% to 100% in the laboratory while in semi-field environments it ranged between 43% to 100% within mortality recording time intervals of 12, 24, 48, and 72 hours. CONCLUSIONS: The larvicidal activity shown by C. osmophloeum leaf essential oil is a promising alternative to existing larvicides or to be incorporated in integrated larval source management compounds for An. gambiae s.s control. The efficacy observed in this study is attributed to both major and minor compounds of the essential oils.
format Online
Article
Text
id pubmed-4019784
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40197842014-05-15 Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s Mdoe, France P Cheng, Sen-Sung Msangi, Shandala Nkwengulila, Gamba Chang, Shang-Tzen Kweka, Eliningaya J Parasit Vectors Research BACKGROUND: The increasing status of insecticide resistant mosquitoes in sub-Saharan Africa is a threatening alert to the existing control efforts. All sibling species of An. gambiae complex have evolved insecticide resistance in wild populations for different approved classes of the insecticides currently in use in the field. An alternative compound for vector control is absolutely urgently needed. In this study, the larvicidal activity and chemical composition of the Cinnamomum osmophloeum leaf essential oils were investigated. METHODS: C. osmophloeum leaf essential oils were extracted by hydrodistillation in a Clevenger-type apparatus for 6 hours, and their chemical compositions identified using GC-MS. These oils were evaluated against An. gambiae s.s. in both laboratory and semi-field situations. The WHO test procedures for monitoring larvicidal efficacy in malaria vectors were used. RESULTS: The composition of C. osmophloeum leaf essential oil has been found to have 11 active compounds. The most abundant compound was trans-cinnamaldehyde (70.20%) and the least abundant was caryophyllene oxide (0.08%). The larvicidal activity was found to be dosage and time dependant both in laboratory and semi-field environments with mortality ranging from 0% to 100%. The LC(50) value was found to vary from 22.18 to 58.15 μg/ml in the laboratory while in semi-field environments it was 11.91 to 63.63 μg/ml. The LC(90) value was found to range between 57.71 to 91.54 μg/ml in the laboratory while in semi-field environments was 52.07 to 173.77 μg/ml. Mortality ranged from 13% to 100% in the laboratory while in semi-field environments it ranged between 43% to 100% within mortality recording time intervals of 12, 24, 48, and 72 hours. CONCLUSIONS: The larvicidal activity shown by C. osmophloeum leaf essential oil is a promising alternative to existing larvicides or to be incorporated in integrated larval source management compounds for An. gambiae s.s control. The efficacy observed in this study is attributed to both major and minor compounds of the essential oils. BioMed Central 2014-05-02 /pmc/articles/PMC4019784/ /pubmed/24885613 http://dx.doi.org/10.1186/1756-3305-7-209 Text en Copyright © 2014 Mdoe et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Mdoe, France P
Cheng, Sen-Sung
Msangi, Shandala
Nkwengulila, Gamba
Chang, Shang-Tzen
Kweka, Eliningaya J
Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title_full Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title_fullStr Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title_full_unstemmed Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title_short Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s
title_sort activity of cinnamomum osmophloeum leaf essential oil against anopheles gambiae s.s
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019784/
https://www.ncbi.nlm.nih.gov/pubmed/24885613
http://dx.doi.org/10.1186/1756-3305-7-209
work_keys_str_mv AT mdoefrancep activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess
AT chengsensung activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess
AT msangishandala activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess
AT nkwengulilagamba activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess
AT changshangtzen activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess
AT kwekaeliningayaj activityofcinnamomumosmophloeumleafessentialoilagainstanophelesgambiaess