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Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease
Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situ microglia. Herein, we present a novel tec...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019954/ https://www.ncbi.nlm.nih.gov/pubmed/24825127 http://dx.doi.org/10.1038/srep04957 |
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author | Ohgidani, Masahiro Kato, Takahiro A. Setoyama, Daiki Sagata, Noriaki Hashimoto, Ryota Shigenobu, Kazue Yoshida, Tetsuhiko Hayakawa, Kohei Shimokawa, Norihiro Miura, Daisuke Utsumi, Hideo Kanba, Shigenobu |
author_facet | Ohgidani, Masahiro Kato, Takahiro A. Setoyama, Daiki Sagata, Noriaki Hashimoto, Ryota Shigenobu, Kazue Yoshida, Tetsuhiko Hayakawa, Kohei Shimokawa, Norihiro Miura, Daisuke Utsumi, Hideo Kanba, Shigenobu |
author_sort | Ohgidani, Masahiro |
collection | PubMed |
description | Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situ microglia. Herein, we present a novel technique for developing induced microglia-like (iMG) cells from human peripheral blood cells. An optimized cocktail of cytokines, GM-CSF and IL-34, converted human monocytes into iMG cells within 14 days. The iMG cells have microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. To confirm clinical utilities, we developed iMG cells from a patient of Nasu-Hakola disease (NHD), which is suggested to be directly caused by microglial dysfunction, and observed that these cells from NHD express delayed but stronger inflammatory responses compared with those from the healthy control. Altogether, the iMG-technique promises to elucidate unresolved aspects of human microglia in various brain disorders. |
format | Online Article Text |
id | pubmed-4019954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-40199542014-05-14 Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease Ohgidani, Masahiro Kato, Takahiro A. Setoyama, Daiki Sagata, Noriaki Hashimoto, Ryota Shigenobu, Kazue Yoshida, Tetsuhiko Hayakawa, Kohei Shimokawa, Norihiro Miura, Daisuke Utsumi, Hideo Kanba, Shigenobu Sci Rep Article Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situ microglia. Herein, we present a novel technique for developing induced microglia-like (iMG) cells from human peripheral blood cells. An optimized cocktail of cytokines, GM-CSF and IL-34, converted human monocytes into iMG cells within 14 days. The iMG cells have microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. To confirm clinical utilities, we developed iMG cells from a patient of Nasu-Hakola disease (NHD), which is suggested to be directly caused by microglial dysfunction, and observed that these cells from NHD express delayed but stronger inflammatory responses compared with those from the healthy control. Altogether, the iMG-technique promises to elucidate unresolved aspects of human microglia in various brain disorders. Nature Publishing Group 2014-05-14 /pmc/articles/PMC4019954/ /pubmed/24825127 http://dx.doi.org/10.1038/srep04957 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Ohgidani, Masahiro Kato, Takahiro A. Setoyama, Daiki Sagata, Noriaki Hashimoto, Ryota Shigenobu, Kazue Yoshida, Tetsuhiko Hayakawa, Kohei Shimokawa, Norihiro Miura, Daisuke Utsumi, Hideo Kanba, Shigenobu Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title | Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title_full | Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title_fullStr | Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title_full_unstemmed | Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title_short | Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease |
title_sort | direct induction of ramified microglia-like cells from human monocytes: dynamic microglial dysfunction in nasu-hakola disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019954/ https://www.ncbi.nlm.nih.gov/pubmed/24825127 http://dx.doi.org/10.1038/srep04957 |
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