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Characterization of the temperature-sensitive reaction of F(1)-ATPase by using single-molecule manipulation
F(1)-ATPase (F(1)) is a rotary motor protein that couples ATP hydrolysis to mechanical rotation with high efficiency. In our recent study, we observed a highly temperature-sensitive (TS) step in the reaction catalyzed by a thermophilic F(1) that was characterized by a rate constant remarkably sensit...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019956/ https://www.ncbi.nlm.nih.gov/pubmed/24825532 http://dx.doi.org/10.1038/srep04962 |