Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored t...

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Autores principales: Merino, Victor R. C., Nakano, Viviane, Finegold, Sydney M., Avila-Campos, Mario J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020208/
https://www.ncbi.nlm.nih.gov/pubmed/24876992
http://dx.doi.org/10.1155/2014/594014
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author Merino, Victor R. C.
Nakano, Viviane
Finegold, Sydney M.
Avila-Campos, Mario J.
author_facet Merino, Victor R. C.
Nakano, Viviane
Finegold, Sydney M.
Avila-Campos, Mario J.
author_sort Merino, Victor R. C.
collection PubMed
description The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.
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spelling pubmed-40202082014-05-29 Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea Merino, Victor R. C. Nakano, Viviane Finegold, Sydney M. Avila-Campos, Mario J. Scientifica (Cairo) Research Article The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains. Hindawi Publishing Corporation 2014 2014-04-29 /pmc/articles/PMC4020208/ /pubmed/24876992 http://dx.doi.org/10.1155/2014/594014 Text en Copyright © 2014 Victor R. C. Merino et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Merino, Victor R. C.
Nakano, Viviane
Finegold, Sydney M.
Avila-Campos, Mario J.
Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_full Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_fullStr Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_full_unstemmed Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_short Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
title_sort genes encoding toxin of clostridium difficile in children with and without diarrhea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020208/
https://www.ncbi.nlm.nih.gov/pubmed/24876992
http://dx.doi.org/10.1155/2014/594014
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