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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA

The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uteru...

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Autores principales: LIU, LIYUAN, WANG, YINGXIONG, YU, QIUBO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020477/
https://www.ncbi.nlm.nih.gov/pubmed/24638941
http://dx.doi.org/10.3892/ijmm.2014.1701
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author LIU, LIYUAN
WANG, YINGXIONG
YU, QIUBO
author_facet LIU, LIYUAN
WANG, YINGXIONG
YU, QIUBO
author_sort LIU, LIYUAN
collection PubMed
description The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.
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spelling pubmed-40204772014-05-14 The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA LIU, LIYUAN WANG, YINGXIONG YU, QIUBO Int J Mol Med Articles The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA. D.A. Spandidos 2014-05 2014-03-17 /pmc/articles/PMC4020477/ /pubmed/24638941 http://dx.doi.org/10.3892/ijmm.2014.1701 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LIU, LIYUAN
WANG, YINGXIONG
YU, QIUBO
The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title_full The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title_fullStr The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title_full_unstemmed The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title_short The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA
title_sort pi3k/akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of rhoa
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020477/
https://www.ncbi.nlm.nih.gov/pubmed/24638941
http://dx.doi.org/10.3892/ijmm.2014.1701
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