Cargando…
Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma
Nasopharyngeal carcinoma (NPC) is one of the most prevalent malignant tumors with poor prognosis in Southern China and Southeast Asia. Angiogenesis-related molecules can be promising therapeutic targets in NPC. To investigate the relationships of cancer-associated fibroblasts (CAFs) and chemokine-re...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020556/ https://www.ncbi.nlm.nih.gov/pubmed/24877105 http://dx.doi.org/10.1155/2014/507353 |
_version_ | 1782316087857119232 |
---|---|
author | Wang, Shumin Ma, Ning Kawanishi, Shosuke Hiraku, Yusuke Oikawa, Shinji Xie, Ying Zhang, Zhe Huang, Guangwu Murata, Mariko |
author_facet | Wang, Shumin Ma, Ning Kawanishi, Shosuke Hiraku, Yusuke Oikawa, Shinji Xie, Ying Zhang, Zhe Huang, Guangwu Murata, Mariko |
author_sort | Wang, Shumin |
collection | PubMed |
description | Nasopharyngeal carcinoma (NPC) is one of the most prevalent malignant tumors with poor prognosis in Southern China and Southeast Asia. Angiogenesis-related molecules can be promising therapeutic targets in NPC. To investigate the relationships of cancer-associated fibroblasts (CAFs) and chemokine-related molecules with neoangiogenesis, we compared immunohistochemical analyses of alpha-smooth-muscle actin (α-SMA), stroma-derived factor-1 (SDF-1), and its receptor CXCR4 in primary NPC specimens and chronic nasopharyngitis tissues. In addition, we examined the expression of vascular endothelial growth factor (VEGF-A), and CD133- and VEGF- receptor-2 (VEGFR-2) double positive cells, as endothelial progenitor cells (EPCs). We also assessed CD34-positive microvessels. Significantly higher expression of α-SMA was observed in fibroblasts in NPC stroma. The immunoreactive intensities of SDF-1 and CXCR4 were significantly higher in NPC cells. CXCR4-positive cells and CD133/VEGFR-2- double positive cells were observed in the stroma surrounding cancer nests, and VEGF was detected in both cancer and stromal cells. Microvessel density was significantly higher in the stroma of NPC tissues compared to chronic nasopharyngitis tissues. Our data suggest that CAFs and NPC tumor cells may enhance neoangiogenesis in a VEGF- and SDF-1-dependent manner by recruiting EPCs from the bone marrow into tumor stroma. |
format | Online Article Text |
id | pubmed-4020556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-40205562014-05-29 Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma Wang, Shumin Ma, Ning Kawanishi, Shosuke Hiraku, Yusuke Oikawa, Shinji Xie, Ying Zhang, Zhe Huang, Guangwu Murata, Mariko Biomed Res Int Research Article Nasopharyngeal carcinoma (NPC) is one of the most prevalent malignant tumors with poor prognosis in Southern China and Southeast Asia. Angiogenesis-related molecules can be promising therapeutic targets in NPC. To investigate the relationships of cancer-associated fibroblasts (CAFs) and chemokine-related molecules with neoangiogenesis, we compared immunohistochemical analyses of alpha-smooth-muscle actin (α-SMA), stroma-derived factor-1 (SDF-1), and its receptor CXCR4 in primary NPC specimens and chronic nasopharyngitis tissues. In addition, we examined the expression of vascular endothelial growth factor (VEGF-A), and CD133- and VEGF- receptor-2 (VEGFR-2) double positive cells, as endothelial progenitor cells (EPCs). We also assessed CD34-positive microvessels. Significantly higher expression of α-SMA was observed in fibroblasts in NPC stroma. The immunoreactive intensities of SDF-1 and CXCR4 were significantly higher in NPC cells. CXCR4-positive cells and CD133/VEGFR-2- double positive cells were observed in the stroma surrounding cancer nests, and VEGF was detected in both cancer and stromal cells. Microvessel density was significantly higher in the stroma of NPC tissues compared to chronic nasopharyngitis tissues. Our data suggest that CAFs and NPC tumor cells may enhance neoangiogenesis in a VEGF- and SDF-1-dependent manner by recruiting EPCs from the bone marrow into tumor stroma. Hindawi Publishing Corporation 2014 2014-04-27 /pmc/articles/PMC4020556/ /pubmed/24877105 http://dx.doi.org/10.1155/2014/507353 Text en Copyright © 2014 Shumin Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Shumin Ma, Ning Kawanishi, Shosuke Hiraku, Yusuke Oikawa, Shinji Xie, Ying Zhang, Zhe Huang, Guangwu Murata, Mariko Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title | Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title_full | Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title_fullStr | Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title_full_unstemmed | Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title_short | Relationships of Alpha-SMA-Positive Fibroblasts and SDF-1-Positive Tumor Cells with Neoangiogenesis in Nasopharyngeal Carcinoma |
title_sort | relationships of alpha-sma-positive fibroblasts and sdf-1-positive tumor cells with neoangiogenesis in nasopharyngeal carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020556/ https://www.ncbi.nlm.nih.gov/pubmed/24877105 http://dx.doi.org/10.1155/2014/507353 |
work_keys_str_mv | AT wangshumin relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT maning relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT kawanishishosuke relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT hirakuyusuke relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT oikawashinji relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT xieying relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT zhangzhe relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT huangguangwu relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma AT muratamariko relationshipsofalphasmapositivefibroblastsandsdf1positivetumorcellswithneoangiogenesisinnasopharyngealcarcinoma |