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Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice
Synphilin-1 is a cytoplasmic protein that has been shown to be involved in the control of energy balance. Previously, we reported on the generation of a human synphilin-1 transgenic mouse model (SP1), in which overexpression of human synphilin-1 resulted in hyperphagia and obesity. Here, behavioral...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020742/ https://www.ncbi.nlm.nih.gov/pubmed/24829096 http://dx.doi.org/10.1371/journal.pone.0091449 |
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author | Li, Xueping Treesukosol, Yada Moghadam, Alexander Smith, Megan Ofeldt, Erica Yang, Dejun Li, Tianxia Tamashiro, Kellie Choi, Pique Moran, Timothy H. Smith, Wanli W. |
author_facet | Li, Xueping Treesukosol, Yada Moghadam, Alexander Smith, Megan Ofeldt, Erica Yang, Dejun Li, Tianxia Tamashiro, Kellie Choi, Pique Moran, Timothy H. Smith, Wanli W. |
author_sort | Li, Xueping |
collection | PubMed |
description | Synphilin-1 is a cytoplasmic protein that has been shown to be involved in the control of energy balance. Previously, we reported on the generation of a human synphilin-1 transgenic mouse model (SP1), in which overexpression of human synphilin-1 resulted in hyperphagia and obesity. Here, behavioral measures in SP1 mice were compared with those of their age-matched controls (NTg) at two time points: when there was not yet a group body weight difference (“pre-obese”) and when SP1 mice were heavier (“obese”). At both time points, meal pattern analyses revealed that SP1 mice displayed higher daily chow intake than non-transgenic control mice. Furthermore, there was an increase in meal size in SP1 mice compared with NTg control mice at the obese stage. In contrast, there was no meal number change between SP1 and NTg control mice. In a brief-access taste procedure, both “pre-obese” and “obese“ SP1 mice displayed concentration-dependent licking across a sucrose concentration range similar to their NTg controls. However, at the pre-obese stage, SP1 mice initiated significantly more trials to sucrose across the testing sessions and licked more vigorously at the highest concentration presented, than the NTg counterparts. These group differences in responsiveness to sucrose were no longer apparent in obese SP1 mice. These results suggest that at the pre-obese stage, the increased trials to sucrose in the SP1 mice reflects increased appetitive behavior to sucrose that may be indicative of the behavioral changes that may contribute to hyperphagia and development of obesity in SP1 mice. These studies provide new insight into synphilin-1 contributions to energy homeostasis. |
format | Online Article Text |
id | pubmed-4020742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40207422014-05-21 Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice Li, Xueping Treesukosol, Yada Moghadam, Alexander Smith, Megan Ofeldt, Erica Yang, Dejun Li, Tianxia Tamashiro, Kellie Choi, Pique Moran, Timothy H. Smith, Wanli W. PLoS One Research Article Synphilin-1 is a cytoplasmic protein that has been shown to be involved in the control of energy balance. Previously, we reported on the generation of a human synphilin-1 transgenic mouse model (SP1), in which overexpression of human synphilin-1 resulted in hyperphagia and obesity. Here, behavioral measures in SP1 mice were compared with those of their age-matched controls (NTg) at two time points: when there was not yet a group body weight difference (“pre-obese”) and when SP1 mice were heavier (“obese”). At both time points, meal pattern analyses revealed that SP1 mice displayed higher daily chow intake than non-transgenic control mice. Furthermore, there was an increase in meal size in SP1 mice compared with NTg control mice at the obese stage. In contrast, there was no meal number change between SP1 and NTg control mice. In a brief-access taste procedure, both “pre-obese” and “obese“ SP1 mice displayed concentration-dependent licking across a sucrose concentration range similar to their NTg controls. However, at the pre-obese stage, SP1 mice initiated significantly more trials to sucrose across the testing sessions and licked more vigorously at the highest concentration presented, than the NTg counterparts. These group differences in responsiveness to sucrose were no longer apparent in obese SP1 mice. These results suggest that at the pre-obese stage, the increased trials to sucrose in the SP1 mice reflects increased appetitive behavior to sucrose that may be indicative of the behavioral changes that may contribute to hyperphagia and development of obesity in SP1 mice. These studies provide new insight into synphilin-1 contributions to energy homeostasis. Public Library of Science 2014-05-14 /pmc/articles/PMC4020742/ /pubmed/24829096 http://dx.doi.org/10.1371/journal.pone.0091449 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Xueping Treesukosol, Yada Moghadam, Alexander Smith, Megan Ofeldt, Erica Yang, Dejun Li, Tianxia Tamashiro, Kellie Choi, Pique Moran, Timothy H. Smith, Wanli W. Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title | Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title_full | Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title_fullStr | Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title_full_unstemmed | Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title_short | Behavioral Characterization of the Hyperphagia Synphilin-1 Overexpressing Mice |
title_sort | behavioral characterization of the hyperphagia synphilin-1 overexpressing mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020742/ https://www.ncbi.nlm.nih.gov/pubmed/24829096 http://dx.doi.org/10.1371/journal.pone.0091449 |
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