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Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique

BACKGROUND: Clinical laboratory reference values from North American and European populations are currently used in most Africans countries due to the absence of locally derived reference ranges, despite previous studies reporting significant differences between populations. Our aim was to define re...

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Autores principales: Tembe, Nelson, Joaquim, Orvalho, Alfai, Eunice, Sitoe, Nádia, Viegas, Edna, Macovela, Eulalia, Gonçalves, Emilia, Osman, Nafissa, Andersson, Sören, Jani, Ilesh, Nilsson, Charlotta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020854/
https://www.ncbi.nlm.nih.gov/pubmed/24827458
http://dx.doi.org/10.1371/journal.pone.0097391
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author Tembe, Nelson
Joaquim, Orvalho
Alfai, Eunice
Sitoe, Nádia
Viegas, Edna
Macovela, Eulalia
Gonçalves, Emilia
Osman, Nafissa
Andersson, Sören
Jani, Ilesh
Nilsson, Charlotta
author_facet Tembe, Nelson
Joaquim, Orvalho
Alfai, Eunice
Sitoe, Nádia
Viegas, Edna
Macovela, Eulalia
Gonçalves, Emilia
Osman, Nafissa
Andersson, Sören
Jani, Ilesh
Nilsson, Charlotta
author_sort Tembe, Nelson
collection PubMed
description BACKGROUND: Clinical laboratory reference values from North American and European populations are currently used in most Africans countries due to the absence of locally derived reference ranges, despite previous studies reporting significant differences between populations. Our aim was to define reference ranges for both genders in 18 to 24 year-old Mozambicans in preparation for clinical vaccine trials. METHODS: A cross-sectional study including 257 volunteers (102 males and 155 females) between 18 and 24 years was performedat a youth clinic in Maputo, Mozambique. All volunteers were clinically healthy and human immunodeficiency virus, Hepatitis B virus and syphilis negative.Median and 95% reference ranges were calculated for immunological, hematological and chemistry parameters. Ranges were compared with those reported based on populations in other African countries and the US. The impact of applying US NIH Division of AIDS (DAIDS) toxicity tables was assessed. RESULTS: The immunology ranges were comparable to those reported for the US and western Kenya.There were significant gender differences in CD4(+) T cell values 713 cells/µL in males versus 824 cells/µL in females (p<0.0001). Hematologic values differed from the US values but were similar to reports of populations in western Kenya and Uganda. The lower and upper limits of the ranges for hemoglobin, hematocrit, red blood cells, white blood cells and lymphocytes were somewhat lower than those from these African countries. The chemistry values were comparable to US values, with few exceptions. The upper limits for ALT, AST, bilirubin, cholesterol and triglycerides were higher than those from the US. DAIDStables for adverse events predicted 297 adverse events and 159 (62%) of the volunteers would have been excluded. CONCLUSION: This study is the first to determine normal laboratory parameters in Mozambique. Our results underscore the necessity of establishing region-specific clinical reference ranges for proper patient management and safe conduct of clinical trials.
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spelling pubmed-40208542014-05-21 Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique Tembe, Nelson Joaquim, Orvalho Alfai, Eunice Sitoe, Nádia Viegas, Edna Macovela, Eulalia Gonçalves, Emilia Osman, Nafissa Andersson, Sören Jani, Ilesh Nilsson, Charlotta PLoS One Research Article BACKGROUND: Clinical laboratory reference values from North American and European populations are currently used in most Africans countries due to the absence of locally derived reference ranges, despite previous studies reporting significant differences between populations. Our aim was to define reference ranges for both genders in 18 to 24 year-old Mozambicans in preparation for clinical vaccine trials. METHODS: A cross-sectional study including 257 volunteers (102 males and 155 females) between 18 and 24 years was performedat a youth clinic in Maputo, Mozambique. All volunteers were clinically healthy and human immunodeficiency virus, Hepatitis B virus and syphilis negative.Median and 95% reference ranges were calculated for immunological, hematological and chemistry parameters. Ranges were compared with those reported based on populations in other African countries and the US. The impact of applying US NIH Division of AIDS (DAIDS) toxicity tables was assessed. RESULTS: The immunology ranges were comparable to those reported for the US and western Kenya.There were significant gender differences in CD4(+) T cell values 713 cells/µL in males versus 824 cells/µL in females (p<0.0001). Hematologic values differed from the US values but were similar to reports of populations in western Kenya and Uganda. The lower and upper limits of the ranges for hemoglobin, hematocrit, red blood cells, white blood cells and lymphocytes were somewhat lower than those from these African countries. The chemistry values were comparable to US values, with few exceptions. The upper limits for ALT, AST, bilirubin, cholesterol and triglycerides were higher than those from the US. DAIDStables for adverse events predicted 297 adverse events and 159 (62%) of the volunteers would have been excluded. CONCLUSION: This study is the first to determine normal laboratory parameters in Mozambique. Our results underscore the necessity of establishing region-specific clinical reference ranges for proper patient management and safe conduct of clinical trials. Public Library of Science 2014-05-14 /pmc/articles/PMC4020854/ /pubmed/24827458 http://dx.doi.org/10.1371/journal.pone.0097391 Text en © 2014 Tembe et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tembe, Nelson
Joaquim, Orvalho
Alfai, Eunice
Sitoe, Nádia
Viegas, Edna
Macovela, Eulalia
Gonçalves, Emilia
Osman, Nafissa
Andersson, Sören
Jani, Ilesh
Nilsson, Charlotta
Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title_full Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title_fullStr Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title_full_unstemmed Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title_short Reference Values for Clinical Laboratory Parameters in Young Adults in Maputo, Mozambique
title_sort reference values for clinical laboratory parameters in young adults in maputo, mozambique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020854/
https://www.ncbi.nlm.nih.gov/pubmed/24827458
http://dx.doi.org/10.1371/journal.pone.0097391
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