Cargando…

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested an...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zhenghui, Li, Xiaoli, He, Xi-Jing, Zhang, Xianghong, Yang, Zhuangqun, Xu, Min, Wu, Baojun, Tu, Junbo, Luo, Huanan, Yan, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020888/
https://www.ncbi.nlm.nih.gov/pubmed/24855367
http://dx.doi.org/10.2147/TCRM.S51518
_version_ 1782316146102370304
author Wang, Zhenghui
Li, Xiaoli
He, Xi-Jing
Zhang, Xianghong
Yang, Zhuangqun
Xu, Min
Wu, Baojun
Tu, Junbo
Luo, Huanan
Yan, Jing
author_facet Wang, Zhenghui
Li, Xiaoli
He, Xi-Jing
Zhang, Xianghong
Yang, Zhuangqun
Xu, Min
Wu, Baojun
Tu, Junbo
Luo, Huanan
Yan, Jing
author_sort Wang, Zhenghui
collection PubMed
description PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested and separately composited with fibrin glue to make the cell suspension, and then transplanted subcutaneously into the back of Sprague Dawley rats after being cultured for 10 days in vitro. Untransplanted animals served as the control group. The allograft and immunological response were examined at 1, 2, 4, 8, and 12 months postoperatively with hematoxylin and eosin histochemical staining, immunohistochemical staining (aggrecan, type II collagen, class I and II major histocompatibility complex), and flow cytometry for peripheral blood cluster of differentiation 4(+) (CD4(+)) and CD8(+) T-cells. RESULTS: There was no infection or death in the rats except one, which died in the first week. Compared to the control group, the RNAi group had fewer eukomonocytes infiltrated, which were only distributed around the graft. The ratio of CD4(+)/CD8(+) T-cells in the RNAi group was significantly lower than the normal one (P<0.05). There were many more positively stained chondrocytes and positively stained areas around the cells in the RNAi group, which were not found in the control group. CONCLUSION: The aggrecanase-1 and aggrecanase-2 RNAi for chondrocytes decreased the immunological rejection effect.
format Online
Article
Text
id pubmed-4020888
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-40208882014-05-22 Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo Wang, Zhenghui Li, Xiaoli He, Xi-Jing Zhang, Xianghong Yang, Zhuangqun Xu, Min Wu, Baojun Tu, Junbo Luo, Huanan Yan, Jing Ther Clin Risk Manag Original Research PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested and separately composited with fibrin glue to make the cell suspension, and then transplanted subcutaneously into the back of Sprague Dawley rats after being cultured for 10 days in vitro. Untransplanted animals served as the control group. The allograft and immunological response were examined at 1, 2, 4, 8, and 12 months postoperatively with hematoxylin and eosin histochemical staining, immunohistochemical staining (aggrecan, type II collagen, class I and II major histocompatibility complex), and flow cytometry for peripheral blood cluster of differentiation 4(+) (CD4(+)) and CD8(+) T-cells. RESULTS: There was no infection or death in the rats except one, which died in the first week. Compared to the control group, the RNAi group had fewer eukomonocytes infiltrated, which were only distributed around the graft. The ratio of CD4(+)/CD8(+) T-cells in the RNAi group was significantly lower than the normal one (P<0.05). There were many more positively stained chondrocytes and positively stained areas around the cells in the RNAi group, which were not found in the control group. CONCLUSION: The aggrecanase-1 and aggrecanase-2 RNAi for chondrocytes decreased the immunological rejection effect. Dove Medical Press 2014-05-08 /pmc/articles/PMC4020888/ /pubmed/24855367 http://dx.doi.org/10.2147/TCRM.S51518 Text en © 2014 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wang, Zhenghui
Li, Xiaoli
He, Xi-Jing
Zhang, Xianghong
Yang, Zhuangqun
Xu, Min
Wu, Baojun
Tu, Junbo
Luo, Huanan
Yan, Jing
Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title_full Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title_fullStr Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title_full_unstemmed Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title_short Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
title_sort experimental study of tissue-engineered cartilage allograft with rnai chondrocytes in vivo
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020888/
https://www.ncbi.nlm.nih.gov/pubmed/24855367
http://dx.doi.org/10.2147/TCRM.S51518
work_keys_str_mv AT wangzhenghui experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT lixiaoli experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT hexijing experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT zhangxianghong experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT yangzhuangqun experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT xumin experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT wubaojun experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT tujunbo experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT luohuanan experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo
AT yanjing experimentalstudyoftissueengineeredcartilageallograftwithrnaichondrocytesinvivo