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Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo
PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020888/ https://www.ncbi.nlm.nih.gov/pubmed/24855367 http://dx.doi.org/10.2147/TCRM.S51518 |
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author | Wang, Zhenghui Li, Xiaoli He, Xi-Jing Zhang, Xianghong Yang, Zhuangqun Xu, Min Wu, Baojun Tu, Junbo Luo, Huanan Yan, Jing |
author_facet | Wang, Zhenghui Li, Xiaoli He, Xi-Jing Zhang, Xianghong Yang, Zhuangqun Xu, Min Wu, Baojun Tu, Junbo Luo, Huanan Yan, Jing |
author_sort | Wang, Zhenghui |
collection | PubMed |
description | PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested and separately composited with fibrin glue to make the cell suspension, and then transplanted subcutaneously into the back of Sprague Dawley rats after being cultured for 10 days in vitro. Untransplanted animals served as the control group. The allograft and immunological response were examined at 1, 2, 4, 8, and 12 months postoperatively with hematoxylin and eosin histochemical staining, immunohistochemical staining (aggrecan, type II collagen, class I and II major histocompatibility complex), and flow cytometry for peripheral blood cluster of differentiation 4(+) (CD4(+)) and CD8(+) T-cells. RESULTS: There was no infection or death in the rats except one, which died in the first week. Compared to the control group, the RNAi group had fewer eukomonocytes infiltrated, which were only distributed around the graft. The ratio of CD4(+)/CD8(+) T-cells in the RNAi group was significantly lower than the normal one (P<0.05). There were many more positively stained chondrocytes and positively stained areas around the cells in the RNAi group, which were not found in the control group. CONCLUSION: The aggrecanase-1 and aggrecanase-2 RNAi for chondrocytes decreased the immunological rejection effect. |
format | Online Article Text |
id | pubmed-4020888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-40208882014-05-22 Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo Wang, Zhenghui Li, Xiaoli He, Xi-Jing Zhang, Xianghong Yang, Zhuangqun Xu, Min Wu, Baojun Tu, Junbo Luo, Huanan Yan, Jing Ther Clin Risk Manag Original Research PURPOSE: To determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds. METHODS: Seven million rat normal and RNAi chondrocytes were harvested and separately composited with fibrin glue to make the cell suspension, and then transplanted subcutaneously into the back of Sprague Dawley rats after being cultured for 10 days in vitro. Untransplanted animals served as the control group. The allograft and immunological response were examined at 1, 2, 4, 8, and 12 months postoperatively with hematoxylin and eosin histochemical staining, immunohistochemical staining (aggrecan, type II collagen, class I and II major histocompatibility complex), and flow cytometry for peripheral blood cluster of differentiation 4(+) (CD4(+)) and CD8(+) T-cells. RESULTS: There was no infection or death in the rats except one, which died in the first week. Compared to the control group, the RNAi group had fewer eukomonocytes infiltrated, which were only distributed around the graft. The ratio of CD4(+)/CD8(+) T-cells in the RNAi group was significantly lower than the normal one (P<0.05). There were many more positively stained chondrocytes and positively stained areas around the cells in the RNAi group, which were not found in the control group. CONCLUSION: The aggrecanase-1 and aggrecanase-2 RNAi for chondrocytes decreased the immunological rejection effect. Dove Medical Press 2014-05-08 /pmc/articles/PMC4020888/ /pubmed/24855367 http://dx.doi.org/10.2147/TCRM.S51518 Text en © 2014 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Wang, Zhenghui Li, Xiaoli He, Xi-Jing Zhang, Xianghong Yang, Zhuangqun Xu, Min Wu, Baojun Tu, Junbo Luo, Huanan Yan, Jing Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title | Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title_full | Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title_fullStr | Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title_full_unstemmed | Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title_short | Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo |
title_sort | experimental study of tissue-engineered cartilage allograft with rnai chondrocytes in vivo |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020888/ https://www.ncbi.nlm.nih.gov/pubmed/24855367 http://dx.doi.org/10.2147/TCRM.S51518 |
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