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A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel

BACKGROUND: Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA). RESULTS: The results showed that the OPA self-assembl...

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Autores principales: Li, Quan-Li, Ning, Tian-Yun, Cao, Ying, Zhang, Wei-bo, Mei, May Lei, Chu, Chun Hung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021083/
https://www.ncbi.nlm.nih.gov/pubmed/24766767
http://dx.doi.org/10.1186/1472-6750-14-32
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author Li, Quan-Li
Ning, Tian-Yun
Cao, Ying
Zhang, Wei-bo
Mei, May Lei
Chu, Chun Hung
author_facet Li, Quan-Li
Ning, Tian-Yun
Cao, Ying
Zhang, Wei-bo
Mei, May Lei
Chu, Chun Hung
author_sort Li, Quan-Li
collection PubMed
description BACKGROUND: Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA). RESULTS: The results showed that the OPA self-assembled into nano-fibres in the presence of calcium ions and in neutral acidity. The OPA was alternately immersed in calcium chloride and sodium hypophosphate solutions to evaluate its property of mineralization. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed nucleation and growth of amorphous calcium phosphate along the self-assembled OPA nano-fibres when it was repetitively exposed to solutions with calcium and phosphate ions. Energy dispersive spectrometry (EDS) confirmed that these nano-particles contained calcium and phosphate. Furthermore, electron diffraction pattern suggested that the nano-particles precipitated on OPA nano-fibres were comparable to amorphous calcium phosphate. Acid-etched human enamel slices were incubated at 37°C in metastable calcium phosphate solution with the OPA for biomimetic mineralization. SEM and X-ray diffraction indicated that the OPA induced the formation of hydroxyapatite crystals in organized bundles on etched enamel. TEM micrographs revealed there were 20–30 nm nano-amorphous calcium phosphate precipitates in the biomimetic mineralizing solution. The particles were found separately bound to the oligopeptide fibres. Biomimetic mineralization with or without the oligopeptide increased demineralized enamel microhardness. CONCLUSIONS: A novel OPA was successfully fabricated, which fostered the biomimetic mineralization of demineralized enamel. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical therapy of dental erosion.
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spelling pubmed-40210832014-05-16 A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel Li, Quan-Li Ning, Tian-Yun Cao, Ying Zhang, Wei-bo Mei, May Lei Chu, Chun Hung BMC Biotechnol Research Article BACKGROUND: Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA). RESULTS: The results showed that the OPA self-assembled into nano-fibres in the presence of calcium ions and in neutral acidity. The OPA was alternately immersed in calcium chloride and sodium hypophosphate solutions to evaluate its property of mineralization. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed nucleation and growth of amorphous calcium phosphate along the self-assembled OPA nano-fibres when it was repetitively exposed to solutions with calcium and phosphate ions. Energy dispersive spectrometry (EDS) confirmed that these nano-particles contained calcium and phosphate. Furthermore, electron diffraction pattern suggested that the nano-particles precipitated on OPA nano-fibres were comparable to amorphous calcium phosphate. Acid-etched human enamel slices were incubated at 37°C in metastable calcium phosphate solution with the OPA for biomimetic mineralization. SEM and X-ray diffraction indicated that the OPA induced the formation of hydroxyapatite crystals in organized bundles on etched enamel. TEM micrographs revealed there were 20–30 nm nano-amorphous calcium phosphate precipitates in the biomimetic mineralizing solution. The particles were found separately bound to the oligopeptide fibres. Biomimetic mineralization with or without the oligopeptide increased demineralized enamel microhardness. CONCLUSIONS: A novel OPA was successfully fabricated, which fostered the biomimetic mineralization of demineralized enamel. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical therapy of dental erosion. BioMed Central 2014-04-27 /pmc/articles/PMC4021083/ /pubmed/24766767 http://dx.doi.org/10.1186/1472-6750-14-32 Text en Copyright © 2014 Li et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Li, Quan-Li
Ning, Tian-Yun
Cao, Ying
Zhang, Wei-bo
Mei, May Lei
Chu, Chun Hung
A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title_full A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title_fullStr A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title_full_unstemmed A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title_short A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
title_sort novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021083/
https://www.ncbi.nlm.nih.gov/pubmed/24766767
http://dx.doi.org/10.1186/1472-6750-14-32
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