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Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism

BACKGROUND: AdpA is a key transcriptional regulator involved in the complex growth cycle of Streptomyces. Streptomyces are Gram-positive bacteria well-known for their production of secondary metabolites and antibiotics. Most work on AdpA has been in S. griseus, and little is known about the pathways...

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Autores principales: Guyet, Aurélie, Benaroudj, Nadia, Proux, Caroline, Gominet, Myriam, Coppée, Jean-Yves, Mazodier, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021200/
https://www.ncbi.nlm.nih.gov/pubmed/24694298
http://dx.doi.org/10.1186/1471-2180-14-81
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author Guyet, Aurélie
Benaroudj, Nadia
Proux, Caroline
Gominet, Myriam
Coppée, Jean-Yves
Mazodier, Philippe
author_facet Guyet, Aurélie
Benaroudj, Nadia
Proux, Caroline
Gominet, Myriam
Coppée, Jean-Yves
Mazodier, Philippe
author_sort Guyet, Aurélie
collection PubMed
description BACKGROUND: AdpA is a key transcriptional regulator involved in the complex growth cycle of Streptomyces. Streptomyces are Gram-positive bacteria well-known for their production of secondary metabolites and antibiotics. Most work on AdpA has been in S. griseus, and little is known about the pathways it controls in other Streptomyces spp. We recently discovered interplay between ClpP peptidases and AdpA in S. lividans. Here, we report the identification of genes directly regulated by AdpA in S. lividans. RESULTS: Microarray experiments revealed that the expression of hundreds of genes was affected in a S. lividans adpA mutant during early stationary phase cultures in YEME liquid medium. We studied the expression of the S. lividans AdpA-regulated genes by quantitative real-time PCR analysis after various times of growth. In silico analysis revealed the presence of potential AdpA-binding sites upstream from these genes; electrophoretic mobility shift assays indicated that AdpA binds directly to their promoter regions. This work identifies new pathways directly controlled by AdpA and that are involved in S. lividans development (ramR, SLI7885 also known as hyaS and SLI6586), and primary (SLI0755-SLI0754 encoding CYP105D5 and Fdx4) or secondary (cchA, cchB, and hyaS) metabolism. CONCLUSIONS: We characterised six S. lividans AdpA-dependent genes whose expression is directly activated by this pleiotropic regulator. Several of these genes are orthologous to bldA-dependent genes in S. coelicolor. Furthermore, in silico analysis suggests that over hundred genes may be directly activated or repressed by S. lividans AdpA, although few have been described as being part of any Streptomyces AdpA regulons. This study increases the number of known AdpA-regulated pathways in Streptomyces spp.
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spelling pubmed-40212002014-05-28 Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism Guyet, Aurélie Benaroudj, Nadia Proux, Caroline Gominet, Myriam Coppée, Jean-Yves Mazodier, Philippe BMC Microbiol Research Article BACKGROUND: AdpA is a key transcriptional regulator involved in the complex growth cycle of Streptomyces. Streptomyces are Gram-positive bacteria well-known for their production of secondary metabolites and antibiotics. Most work on AdpA has been in S. griseus, and little is known about the pathways it controls in other Streptomyces spp. We recently discovered interplay between ClpP peptidases and AdpA in S. lividans. Here, we report the identification of genes directly regulated by AdpA in S. lividans. RESULTS: Microarray experiments revealed that the expression of hundreds of genes was affected in a S. lividans adpA mutant during early stationary phase cultures in YEME liquid medium. We studied the expression of the S. lividans AdpA-regulated genes by quantitative real-time PCR analysis after various times of growth. In silico analysis revealed the presence of potential AdpA-binding sites upstream from these genes; electrophoretic mobility shift assays indicated that AdpA binds directly to their promoter regions. This work identifies new pathways directly controlled by AdpA and that are involved in S. lividans development (ramR, SLI7885 also known as hyaS and SLI6586), and primary (SLI0755-SLI0754 encoding CYP105D5 and Fdx4) or secondary (cchA, cchB, and hyaS) metabolism. CONCLUSIONS: We characterised six S. lividans AdpA-dependent genes whose expression is directly activated by this pleiotropic regulator. Several of these genes are orthologous to bldA-dependent genes in S. coelicolor. Furthermore, in silico analysis suggests that over hundred genes may be directly activated or repressed by S. lividans AdpA, although few have been described as being part of any Streptomyces AdpA regulons. This study increases the number of known AdpA-regulated pathways in Streptomyces spp. BioMed Central 2014-04-03 /pmc/articles/PMC4021200/ /pubmed/24694298 http://dx.doi.org/10.1186/1471-2180-14-81 Text en Copyright © 2014 Guyet et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Guyet, Aurélie
Benaroudj, Nadia
Proux, Caroline
Gominet, Myriam
Coppée, Jean-Yves
Mazodier, Philippe
Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title_full Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title_fullStr Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title_full_unstemmed Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title_short Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism
title_sort identified members of the streptomyces lividans adpa regulon involved in differentiation and secondary metabolism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021200/
https://www.ncbi.nlm.nih.gov/pubmed/24694298
http://dx.doi.org/10.1186/1471-2180-14-81
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