Cargando…

A reduced genome decreases the host carrying capacity for foreign DNA

BACKGROUND: Host-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called “clean genome” (i.e., MDS42) of reduced genome size has re...

Descripción completa

Detalles Bibliográficos
Autores principales: Akeno, Yuya, Ying, Bei-Wen, Tsuru, Saburo, Yomo, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021254/
https://www.ncbi.nlm.nih.gov/pubmed/24685185
http://dx.doi.org/10.1186/1475-2859-13-49
_version_ 1782316203887296512
author Akeno, Yuya
Ying, Bei-Wen
Tsuru, Saburo
Yomo, Tetsuya
author_facet Akeno, Yuya
Ying, Bei-Wen
Tsuru, Saburo
Yomo, Tetsuya
author_sort Akeno, Yuya
collection PubMed
description BACKGROUND: Host-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called “clean genome” (i.e., MDS42) of reduced genome size has recently been generated from the wild-type strain MG1655, a commonly used host strain. A quantitative evaluation of the influence of plasmid burdens in these two Escherichia coli strains can not only provide an understanding of how a reduced genome responds to foreign DNA but also offer insights into the proper application of these strains. RESULTS: The decreases in growth caused by the cost of carrying foreign DNA were similar for the wild-type and clean-genome strains. A negative correlation between the growth rate and the total amount of exogenous DNA was observed in both strains, but a better theoretical fit with a higher statistical significance was found for the strain with the clean genome. Compared to the wild-type strain, the clean-genome strain exhibited a reduced carrying capacity for exogenous DNA, which was largely attributed to its ability to restrict the replication of foreign DNA. A tendency to allocate energy and resources toward gene expression, but not DNA replication, was observed in the strain with the clean genome. CONCLUSIONS: The possession of a clean genome constrained the plasmid copy number to a wild-type-equivalent load. The results indicate that the wild-type strain possesses a greater tolerance for foreign DNA, as in endosymbiosis, and that the use of strains with clean genomes will be favorable in the applications that require precise control and theoretical prediction.
format Online
Article
Text
id pubmed-4021254
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40212542014-05-28 A reduced genome decreases the host carrying capacity for foreign DNA Akeno, Yuya Ying, Bei-Wen Tsuru, Saburo Yomo, Tetsuya Microb Cell Fact Research BACKGROUND: Host-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called “clean genome” (i.e., MDS42) of reduced genome size has recently been generated from the wild-type strain MG1655, a commonly used host strain. A quantitative evaluation of the influence of plasmid burdens in these two Escherichia coli strains can not only provide an understanding of how a reduced genome responds to foreign DNA but also offer insights into the proper application of these strains. RESULTS: The decreases in growth caused by the cost of carrying foreign DNA were similar for the wild-type and clean-genome strains. A negative correlation between the growth rate and the total amount of exogenous DNA was observed in both strains, but a better theoretical fit with a higher statistical significance was found for the strain with the clean genome. Compared to the wild-type strain, the clean-genome strain exhibited a reduced carrying capacity for exogenous DNA, which was largely attributed to its ability to restrict the replication of foreign DNA. A tendency to allocate energy and resources toward gene expression, but not DNA replication, was observed in the strain with the clean genome. CONCLUSIONS: The possession of a clean genome constrained the plasmid copy number to a wild-type-equivalent load. The results indicate that the wild-type strain possesses a greater tolerance for foreign DNA, as in endosymbiosis, and that the use of strains with clean genomes will be favorable in the applications that require precise control and theoretical prediction. BioMed Central 2014-04-01 /pmc/articles/PMC4021254/ /pubmed/24685185 http://dx.doi.org/10.1186/1475-2859-13-49 Text en Copyright © 2014 Akeno et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Akeno, Yuya
Ying, Bei-Wen
Tsuru, Saburo
Yomo, Tetsuya
A reduced genome decreases the host carrying capacity for foreign DNA
title A reduced genome decreases the host carrying capacity for foreign DNA
title_full A reduced genome decreases the host carrying capacity for foreign DNA
title_fullStr A reduced genome decreases the host carrying capacity for foreign DNA
title_full_unstemmed A reduced genome decreases the host carrying capacity for foreign DNA
title_short A reduced genome decreases the host carrying capacity for foreign DNA
title_sort reduced genome decreases the host carrying capacity for foreign dna
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021254/
https://www.ncbi.nlm.nih.gov/pubmed/24685185
http://dx.doi.org/10.1186/1475-2859-13-49
work_keys_str_mv AT akenoyuya areducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT yingbeiwen areducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT tsurusaburo areducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT yomotetsuya areducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT akenoyuya reducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT yingbeiwen reducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT tsurusaburo reducedgenomedecreasesthehostcarryingcapacityforforeigndna
AT yomotetsuya reducedgenomedecreasesthehostcarryingcapacityforforeigndna