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A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility

BACKGROUND: The MADS-box transcription factors play fundamental roles in reproductive developmental control. Although the roles of many plant MADS-box proteins have been extensively studied, there are almost no functional studies of them in soybean, an important protein and oil crop in the world. In...

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Autores principales: Huang, Fang, Xu, Guangli, Chi, Yingjun, Liu, Haicui, Xue, Qian, Zhao, Tuanjie, Gai, Junyi, Yu, Deyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021551/
https://www.ncbi.nlm.nih.gov/pubmed/24693922
http://dx.doi.org/10.1186/1471-2229-14-89
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author Huang, Fang
Xu, Guangli
Chi, Yingjun
Liu, Haicui
Xue, Qian
Zhao, Tuanjie
Gai, Junyi
Yu, Deyue
author_facet Huang, Fang
Xu, Guangli
Chi, Yingjun
Liu, Haicui
Xue, Qian
Zhao, Tuanjie
Gai, Junyi
Yu, Deyue
author_sort Huang, Fang
collection PubMed
description BACKGROUND: The MADS-box transcription factors play fundamental roles in reproductive developmental control. Although the roles of many plant MADS-box proteins have been extensively studied, there are almost no functional studies of them in soybean, an important protein and oil crop in the world. In addition, the MADS-box protein orthologs may have species-specific functions. Controlling male fertility is an important goal in plant hybrid breeding but is difficult in some crops like soybean. The morphological structure of soybean flowers prevents the cross-pollination. Understanding the molecular mechanisms for floral development will aid in engineering new sterile materials that could be applied in hybrid breeding programs in soybean. RESULT: Through microarray analysis, a flower-enriched gene in soybean was selected and designated as GmMADS28. GmMADS28 belongs to AGL9/SEP subfamily of MADS-box proteins, localized in nucleus and showed specific expression patterns in floral meristems as well as stamen and petal primordia. Expression of GmMADS28 in the stamens and petals of a soybean mutant NJS-10Hfs whose stamens are converted into petals was higher than in those of wild-type plants. Constitutive expression of GmMADS28 in tobacco promoted early flowering and converted stamens and sepals to petals. Interestingly, transgenic plants increased the numbers of sepal, petal and stamen from five to six and exhibited male sterility due to the shortened and curly filaments and the failure of pollen release from the anthers. The ectopic expression of GmMADS28 was found to be sufficient to activate expression of tobacco homologs of SOC1, LEAFY, AGL8/FUL, and DEF. In addition, we observed the interactions of GmMADS28 with soybean homologs of SOC1, AP1, and AGL8/FUL proteins. CONCLUSION: In this study, we observed the roles of GmMADS28 in the regulation of floral organ number and petal identity. Compared to other plant AGL9/SEP proteins, GmMADS28 specifically regulates floral organ number, filament length and pollen release. The sterility caused by the ectopic expression of GmMADS28 offers a promising way to genetically produce new sterile material that could potentially be applied in the hybrid breeding of crops like soybean.
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spelling pubmed-40215512014-05-16 A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility Huang, Fang Xu, Guangli Chi, Yingjun Liu, Haicui Xue, Qian Zhao, Tuanjie Gai, Junyi Yu, Deyue BMC Plant Biol Research Article BACKGROUND: The MADS-box transcription factors play fundamental roles in reproductive developmental control. Although the roles of many plant MADS-box proteins have been extensively studied, there are almost no functional studies of them in soybean, an important protein and oil crop in the world. In addition, the MADS-box protein orthologs may have species-specific functions. Controlling male fertility is an important goal in plant hybrid breeding but is difficult in some crops like soybean. The morphological structure of soybean flowers prevents the cross-pollination. Understanding the molecular mechanisms for floral development will aid in engineering new sterile materials that could be applied in hybrid breeding programs in soybean. RESULT: Through microarray analysis, a flower-enriched gene in soybean was selected and designated as GmMADS28. GmMADS28 belongs to AGL9/SEP subfamily of MADS-box proteins, localized in nucleus and showed specific expression patterns in floral meristems as well as stamen and petal primordia. Expression of GmMADS28 in the stamens and petals of a soybean mutant NJS-10Hfs whose stamens are converted into petals was higher than in those of wild-type plants. Constitutive expression of GmMADS28 in tobacco promoted early flowering and converted stamens and sepals to petals. Interestingly, transgenic plants increased the numbers of sepal, petal and stamen from five to six and exhibited male sterility due to the shortened and curly filaments and the failure of pollen release from the anthers. The ectopic expression of GmMADS28 was found to be sufficient to activate expression of tobacco homologs of SOC1, LEAFY, AGL8/FUL, and DEF. In addition, we observed the interactions of GmMADS28 with soybean homologs of SOC1, AP1, and AGL8/FUL proteins. CONCLUSION: In this study, we observed the roles of GmMADS28 in the regulation of floral organ number and petal identity. Compared to other plant AGL9/SEP proteins, GmMADS28 specifically regulates floral organ number, filament length and pollen release. The sterility caused by the ectopic expression of GmMADS28 offers a promising way to genetically produce new sterile material that could potentially be applied in the hybrid breeding of crops like soybean. BioMed Central 2014-04-02 /pmc/articles/PMC4021551/ /pubmed/24693922 http://dx.doi.org/10.1186/1471-2229-14-89 Text en Copyright © 2014 Huang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Fang
Xu, Guangli
Chi, Yingjun
Liu, Haicui
Xue, Qian
Zhao, Tuanjie
Gai, Junyi
Yu, Deyue
A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title_full A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title_fullStr A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title_full_unstemmed A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title_short A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility
title_sort soybean mads-box protein modulates floral organ numbers, petal identity and sterility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021551/
https://www.ncbi.nlm.nih.gov/pubmed/24693922
http://dx.doi.org/10.1186/1471-2229-14-89
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