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A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration
Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold,...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022101/ https://www.ncbi.nlm.nih.gov/pubmed/22305104 http://dx.doi.org/10.1016/j.biomaterials.2012.01.012 |
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| author | Totonelli, Giorgia Maghsoudlou, Panagiotis Garriboli, Massimo Riegler, Johannes Orlando, Giuseppe Burns, Alan J. Sebire, Neil J. Smith, Virpi V. Fishman, Jonathan M. Ghionzoli, Marco Turmaine, Mark Birchall, Martin A. Atala, Anthony Soker, Shay Lythgoe, Mark F. Seifalian, Alexander Pierro, Agostino Eaton, Simon De Coppi, Paolo |
| author_facet | Totonelli, Giorgia Maghsoudlou, Panagiotis Garriboli, Massimo Riegler, Johannes Orlando, Giuseppe Burns, Alan J. Sebire, Neil J. Smith, Virpi V. Fishman, Jonathan M. Ghionzoli, Marco Turmaine, Mark Birchall, Martin A. Atala, Anthony Soker, Shay Lythgoe, Mark F. Seifalian, Alexander Pierro, Agostino Eaton, Simon De Coppi, Paolo |
| author_sort | Totonelli, Giorgia |
| collection | PubMed |
| description | Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration. |
| format | Online Article Text |
| id | pubmed-4022101 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| publisher | Elsevier Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-40221012014-05-19 A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration Totonelli, Giorgia Maghsoudlou, Panagiotis Garriboli, Massimo Riegler, Johannes Orlando, Giuseppe Burns, Alan J. Sebire, Neil J. Smith, Virpi V. Fishman, Jonathan M. Ghionzoli, Marco Turmaine, Mark Birchall, Martin A. Atala, Anthony Soker, Shay Lythgoe, Mark F. Seifalian, Alexander Pierro, Agostino Eaton, Simon De Coppi, Paolo Biomaterials Article Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration. Elsevier Science 2012-04 /pmc/articles/PMC4022101/ /pubmed/22305104 http://dx.doi.org/10.1016/j.biomaterials.2012.01.012 Text en © 2012 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
| spellingShingle | Article Totonelli, Giorgia Maghsoudlou, Panagiotis Garriboli, Massimo Riegler, Johannes Orlando, Giuseppe Burns, Alan J. Sebire, Neil J. Smith, Virpi V. Fishman, Jonathan M. Ghionzoli, Marco Turmaine, Mark Birchall, Martin A. Atala, Anthony Soker, Shay Lythgoe, Mark F. Seifalian, Alexander Pierro, Agostino Eaton, Simon De Coppi, Paolo A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title | A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title_full | A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title_fullStr | A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title_full_unstemmed | A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title_short | A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| title_sort | rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022101/ https://www.ncbi.nlm.nih.gov/pubmed/22305104 http://dx.doi.org/10.1016/j.biomaterials.2012.01.012 |
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