Cargando…

In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang

Nefang, a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), is a potential therapy against P. falciparum malaria. In vitro antiplasmodial activities of its constituent solvent extrac...

Descripción completa

Detalles Bibliográficos
Autores principales: Arrey Tarkang, Protus, Franzoi, Kathrin Diehl, Lee, Sukjun, Lee, Eunyoung, Vivarelli, Diego, Freitas-Junior, Lucio, Liuzzi, Michel, Nolé, Tsabang, Ayong, Lawrence S., Agbor, Gabriel A., Okalebo, Faith A., Guantai, Anastasia N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022163/
https://www.ncbi.nlm.nih.gov/pubmed/24877138
http://dx.doi.org/10.1155/2014/835013
_version_ 1782316357095784448
author Arrey Tarkang, Protus
Franzoi, Kathrin Diehl
Lee, Sukjun
Lee, Eunyoung
Vivarelli, Diego
Freitas-Junior, Lucio
Liuzzi, Michel
Nolé, Tsabang
Ayong, Lawrence S.
Agbor, Gabriel A.
Okalebo, Faith A.
Guantai, Anastasia N.
author_facet Arrey Tarkang, Protus
Franzoi, Kathrin Diehl
Lee, Sukjun
Lee, Eunyoung
Vivarelli, Diego
Freitas-Junior, Lucio
Liuzzi, Michel
Nolé, Tsabang
Ayong, Lawrence S.
Agbor, Gabriel A.
Okalebo, Faith A.
Guantai, Anastasia N.
author_sort Arrey Tarkang, Protus
collection PubMed
description Nefang, a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), is a potential therapy against P. falciparum malaria. In vitro antiplasmodial activities of its constituent solvent extracts were analyzed on CQ-sensitive (3D7) and multidrug resistant (Dd2) P. falciparum strains. The interactions involving the differential solvent extracts were further analyzed using a variable potency ratio drug combination approach. Effective concentration 50 (EC(50)) values were determined by nonlinear regression curve-fitting of the dose-response data and used in calculating the fractional inhibitory concentration 50 (FIC(50)) and combination indices (CI) for each pair. The derived EC(50) values (3D7/Dd2, μg/mL) are Nefang-96.96/55.08, MiB-65.33/34.58, MiL-82.56/40.04, Pg-47.02/25.79, Cp-1188/317.5, Cc-723.3/141, Cs-184.4/105.1, and Og-778.5/118.9. Synergism was obtained with MiB/Pg (CI = 0.351), MiL/Pg (0.358), MiB/Cs (0.366), MiL/Cs (0.482), Pg/Cs (0.483), and Cs/Og (0.414) when analyzed at equipotency ratios. Cytotoxicity testing of Nefang and the solvent extracts on two human cell lines (Hep G2 and U2OS) revealed no significant toxicity relative to their antiplasmodial activities (SI > 20). Taken together, our data confirm the antimalarial activities of Nefang and its constituent plant extracts and identified extract pairs with promising synergistic interactions for exploitation towards a rational phytotherapeutic and evidence-based antimalarial drug discovery.
format Online
Article
Text
id pubmed-4022163
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-40221632014-05-29 In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang Arrey Tarkang, Protus Franzoi, Kathrin Diehl Lee, Sukjun Lee, Eunyoung Vivarelli, Diego Freitas-Junior, Lucio Liuzzi, Michel Nolé, Tsabang Ayong, Lawrence S. Agbor, Gabriel A. Okalebo, Faith A. Guantai, Anastasia N. Biomed Res Int Research Article Nefang, a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), is a potential therapy against P. falciparum malaria. In vitro antiplasmodial activities of its constituent solvent extracts were analyzed on CQ-sensitive (3D7) and multidrug resistant (Dd2) P. falciparum strains. The interactions involving the differential solvent extracts were further analyzed using a variable potency ratio drug combination approach. Effective concentration 50 (EC(50)) values were determined by nonlinear regression curve-fitting of the dose-response data and used in calculating the fractional inhibitory concentration 50 (FIC(50)) and combination indices (CI) for each pair. The derived EC(50) values (3D7/Dd2, μg/mL) are Nefang-96.96/55.08, MiB-65.33/34.58, MiL-82.56/40.04, Pg-47.02/25.79, Cp-1188/317.5, Cc-723.3/141, Cs-184.4/105.1, and Og-778.5/118.9. Synergism was obtained with MiB/Pg (CI = 0.351), MiL/Pg (0.358), MiB/Cs (0.366), MiL/Cs (0.482), Pg/Cs (0.483), and Cs/Og (0.414) when analyzed at equipotency ratios. Cytotoxicity testing of Nefang and the solvent extracts on two human cell lines (Hep G2 and U2OS) revealed no significant toxicity relative to their antiplasmodial activities (SI > 20). Taken together, our data confirm the antimalarial activities of Nefang and its constituent plant extracts and identified extract pairs with promising synergistic interactions for exploitation towards a rational phytotherapeutic and evidence-based antimalarial drug discovery. Hindawi Publishing Corporation 2014 2014-04-27 /pmc/articles/PMC4022163/ /pubmed/24877138 http://dx.doi.org/10.1155/2014/835013 Text en Copyright © 2014 Protus Arrey Tarkang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arrey Tarkang, Protus
Franzoi, Kathrin Diehl
Lee, Sukjun
Lee, Eunyoung
Vivarelli, Diego
Freitas-Junior, Lucio
Liuzzi, Michel
Nolé, Tsabang
Ayong, Lawrence S.
Agbor, Gabriel A.
Okalebo, Faith A.
Guantai, Anastasia N.
In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title_full In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title_fullStr In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title_full_unstemmed In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title_short In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
title_sort in vitro antiplasmodial activities and synergistic combinations of differential solvent extracts of the polyherbal product, nefang
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022163/
https://www.ncbi.nlm.nih.gov/pubmed/24877138
http://dx.doi.org/10.1155/2014/835013
work_keys_str_mv AT arreytarkangprotus invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT franzoikathrindiehl invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT leesukjun invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT leeeunyoung invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT vivarellidiego invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT freitasjuniorlucio invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT liuzzimichel invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT noletsabang invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT ayonglawrences invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT agborgabriela invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT okalebofaitha invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang
AT guantaianastasian invitroantiplasmodialactivitiesandsynergisticcombinationsofdifferentialsolventextractsofthepolyherbalproductnefang