Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-...

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Autores principales: Kamboj, Aman, Pateriya, Atul Kumar, Mishra, Anamika, Ranaware, Pradip, Kulkarni, Diwakar D., Raut, Ashwin Ashok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022296/
https://www.ncbi.nlm.nih.gov/pubmed/24877102
http://dx.doi.org/10.1155/2014/496219
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author Kamboj, Aman
Pateriya, Atul Kumar
Mishra, Anamika
Ranaware, Pradip
Kulkarni, Diwakar D.
Raut, Ashwin Ashok
author_facet Kamboj, Aman
Pateriya, Atul Kumar
Mishra, Anamika
Ranaware, Pradip
Kulkarni, Diwakar D.
Raut, Ashwin Ashok
author_sort Kamboj, Aman
collection PubMed
description Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.
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spelling pubmed-40222962014-05-29 Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India Kamboj, Aman Pateriya, Atul Kumar Mishra, Anamika Ranaware, Pradip Kulkarni, Diwakar D. Raut, Ashwin Ashok Biomed Res Int Research Article Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF. Hindawi Publishing Corporation 2014 2014-04-27 /pmc/articles/PMC4022296/ /pubmed/24877102 http://dx.doi.org/10.1155/2014/496219 Text en Copyright © 2014 Aman Kamboj et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kamboj, Aman
Pateriya, Atul Kumar
Mishra, Anamika
Ranaware, Pradip
Kulkarni, Diwakar D.
Raut, Ashwin Ashok
Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_full Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_fullStr Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_full_unstemmed Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_short Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_sort novel molecular beacon probe-based real-time rt-pcr assay for diagnosis of crimean-congo hemorrhagic fever encountered in india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022296/
https://www.ncbi.nlm.nih.gov/pubmed/24877102
http://dx.doi.org/10.1155/2014/496219
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