Cargando…

Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons

BACKGROUND: Action potentials can be initiated at various subcellular compartments, such as axonal hillock, soma and dendrite. Mechanisms and physiological impacts for this relocation remain elusive, which may rely on input signal patterns and intrinsic properties in these subcellular compartments....

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Rongjing, Qian, Hao, Chen, Na, Wang, Jin-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022375/
https://www.ncbi.nlm.nih.gov/pubmed/24708847
http://dx.doi.org/10.1186/1756-6606-7-26
_version_ 1782316392346812416
author Ge, Rongjing
Qian, Hao
Chen, Na
Wang, Jin-Hui
author_facet Ge, Rongjing
Qian, Hao
Chen, Na
Wang, Jin-Hui
author_sort Ge, Rongjing
collection PubMed
description BACKGROUND: Action potentials can be initiated at various subcellular compartments, such as axonal hillock, soma and dendrite. Mechanisms and physiological impacts for this relocation remain elusive, which may rely on input signal patterns and intrinsic properties in these subcellular compartments. We examined this hypothesis at the soma and axon of cortical pyramidal neurons by analyzing their spike capability and voltage-gated sodium channel dynamics in response to different input signals. RESULTS: Electrophysiological recordings were simultaneously conducted at the somata and axons of identical pyramidal neurons in the cortical slices. The somata dominantly produced sequential spikes in response to long-time steady depolarization pulse, and the axons produced more spikes in response to fluctuated pulse. Compared with the axons, the somata possessed lower spike threshold and shorter refractory periods in response to long-time steady depolarization, and somatic voltage-gated sodium channels demonstrated less inactivation and easier reactivation in response to steady depolarization. Based on local VGSC dynamics, computational simulated spike initiation locations were consistent with those from the experiments. In terms of physiological impact, this input-dependent plasticity of spike initiation location made neuronal encoding to be efficient. CONCLUSIONS: Long-time steady depolarization primarily induces somatic spikes and short-time pulses induce axonal spikes. The input signal patterns influence spike initiations at the axon or soma of cortical pyramidal neurons through modulating local voltage-gated sodium channel dynamics.
format Online
Article
Text
id pubmed-4022375
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40223752014-05-28 Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons Ge, Rongjing Qian, Hao Chen, Na Wang, Jin-Hui Mol Brain Research BACKGROUND: Action potentials can be initiated at various subcellular compartments, such as axonal hillock, soma and dendrite. Mechanisms and physiological impacts for this relocation remain elusive, which may rely on input signal patterns and intrinsic properties in these subcellular compartments. We examined this hypothesis at the soma and axon of cortical pyramidal neurons by analyzing their spike capability and voltage-gated sodium channel dynamics in response to different input signals. RESULTS: Electrophysiological recordings were simultaneously conducted at the somata and axons of identical pyramidal neurons in the cortical slices. The somata dominantly produced sequential spikes in response to long-time steady depolarization pulse, and the axons produced more spikes in response to fluctuated pulse. Compared with the axons, the somata possessed lower spike threshold and shorter refractory periods in response to long-time steady depolarization, and somatic voltage-gated sodium channels demonstrated less inactivation and easier reactivation in response to steady depolarization. Based on local VGSC dynamics, computational simulated spike initiation locations were consistent with those from the experiments. In terms of physiological impact, this input-dependent plasticity of spike initiation location made neuronal encoding to be efficient. CONCLUSIONS: Long-time steady depolarization primarily induces somatic spikes and short-time pulses induce axonal spikes. The input signal patterns influence spike initiations at the axon or soma of cortical pyramidal neurons through modulating local voltage-gated sodium channel dynamics. BioMed Central 2014-04-04 /pmc/articles/PMC4022375/ /pubmed/24708847 http://dx.doi.org/10.1186/1756-6606-7-26 Text en Copyright © 2014 Ge et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ge, Rongjing
Qian, Hao
Chen, Na
Wang, Jin-Hui
Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title_full Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title_fullStr Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title_full_unstemmed Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title_short Input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
title_sort input-dependent subcellular localization of spike initiation between soma and axon at cortical pyramidal neurons
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022375/
https://www.ncbi.nlm.nih.gov/pubmed/24708847
http://dx.doi.org/10.1186/1756-6606-7-26
work_keys_str_mv AT gerongjing inputdependentsubcellularlocalizationofspikeinitiationbetweensomaandaxonatcorticalpyramidalneurons
AT qianhao inputdependentsubcellularlocalizationofspikeinitiationbetweensomaandaxonatcorticalpyramidalneurons
AT chenna inputdependentsubcellularlocalizationofspikeinitiationbetweensomaandaxonatcorticalpyramidalneurons
AT wangjinhui inputdependentsubcellularlocalizationofspikeinitiationbetweensomaandaxonatcorticalpyramidalneurons