Cargando…

Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector

BACKGROUND: Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of inte...

Descripción completa

Detalles Bibliográficos
Autores principales: Hammac, G Kenitra, Pierlé, Sebastián Aguilar, Cheng, Xiaoya, Scoles, Glen A, Brayton, Kelly A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022386/
https://www.ncbi.nlm.nih.gov/pubmed/24751137
http://dx.doi.org/10.1186/1756-3305-7-193
_version_ 1782316394875977728
author Hammac, G Kenitra
Pierlé, Sebastián Aguilar
Cheng, Xiaoya
Scoles, Glen A
Brayton, Kelly A
author_facet Hammac, G Kenitra
Pierlé, Sebastián Aguilar
Cheng, Xiaoya
Scoles, Glen A
Brayton, Kelly A
author_sort Hammac, G Kenitra
collection PubMed
description BACKGROUND: Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale, the tick-borne causative agent of bovine anaplasmosis. METHODS: RNA-seq technology allowed a comprehensive analysis of the transcriptional status of A. marginale genes in two conditions: bovine host blood and tick derived cell culture, a model for the tick vector. Quantitative PCR was used to assess transcription of a set of genes in A. marginale infected tick midguts and salivary glands at two time points during the transmission cycle. RESULTS: Genes belonging to fourteen pathways or component groups were found to be differentially transcribed in A. marginale in the bovine host versus the tick vector. One of the most significantly altered groups was composed of surface proteins. Of the 56 genes included in the surface protein group, eight were up regulated and 26 were down regulated. The down regulated surface protein encoding genes include several that are well studied due to their immunogenicity and function. Quantitative PCR of a set of genes demonstrated that transcription in tick cell culture most closely approximates transcription in salivary glands of recently infected ticks. CONCLUSIONS: The ISE6 tick cell culture line is an acceptable model for early infection in tick salivary glands, and reveals disproportionate down regulation of surface protein genes in the tick. Transcriptional profiling in other cell lines may help us simulate additional microenvironments. Understanding vector-specific alteration of gene transcription, especially of surface protein encoding genes, may aid in the development of vaccines or transmission blocking therapies.
format Online
Article
Text
id pubmed-4022386
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40223862014-05-16 Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector Hammac, G Kenitra Pierlé, Sebastián Aguilar Cheng, Xiaoya Scoles, Glen A Brayton, Kelly A Parasit Vectors Research BACKGROUND: Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale, the tick-borne causative agent of bovine anaplasmosis. METHODS: RNA-seq technology allowed a comprehensive analysis of the transcriptional status of A. marginale genes in two conditions: bovine host blood and tick derived cell culture, a model for the tick vector. Quantitative PCR was used to assess transcription of a set of genes in A. marginale infected tick midguts and salivary glands at two time points during the transmission cycle. RESULTS: Genes belonging to fourteen pathways or component groups were found to be differentially transcribed in A. marginale in the bovine host versus the tick vector. One of the most significantly altered groups was composed of surface proteins. Of the 56 genes included in the surface protein group, eight were up regulated and 26 were down regulated. The down regulated surface protein encoding genes include several that are well studied due to their immunogenicity and function. Quantitative PCR of a set of genes demonstrated that transcription in tick cell culture most closely approximates transcription in salivary glands of recently infected ticks. CONCLUSIONS: The ISE6 tick cell culture line is an acceptable model for early infection in tick salivary glands, and reveals disproportionate down regulation of surface protein genes in the tick. Transcriptional profiling in other cell lines may help us simulate additional microenvironments. Understanding vector-specific alteration of gene transcription, especially of surface protein encoding genes, may aid in the development of vaccines or transmission blocking therapies. BioMed Central 2014-04-21 /pmc/articles/PMC4022386/ /pubmed/24751137 http://dx.doi.org/10.1186/1756-3305-7-193 Text en Copyright © 2014 Hammac et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hammac, G Kenitra
Pierlé, Sebastián Aguilar
Cheng, Xiaoya
Scoles, Glen A
Brayton, Kelly A
Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title_full Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title_fullStr Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title_full_unstemmed Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title_short Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
title_sort global transcriptional analysis reveals surface remodeling of anaplasma marginale in the tick vector
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022386/
https://www.ncbi.nlm.nih.gov/pubmed/24751137
http://dx.doi.org/10.1186/1756-3305-7-193
work_keys_str_mv AT hammacgkenitra globaltranscriptionalanalysisrevealssurfaceremodelingofanaplasmamarginaleinthetickvector
AT pierlesebastianaguilar globaltranscriptionalanalysisrevealssurfaceremodelingofanaplasmamarginaleinthetickvector
AT chengxiaoya globaltranscriptionalanalysisrevealssurfaceremodelingofanaplasmamarginaleinthetickvector
AT scolesglena globaltranscriptionalanalysisrevealssurfaceremodelingofanaplasmamarginaleinthetickvector
AT braytonkellya globaltranscriptionalanalysisrevealssurfaceremodelingofanaplasmamarginaleinthetickvector