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High-Throughput Screening of Effective siRNAs Using Luciferase-Linked Chimeric mRNA

The use of siRNAs to knock down gene expression can potentially be an approach to treat various diseases. To avoid siRNA toxicity the less transcriptionally active H1 pol III promoter, rather than the U6 promoter, was proposed for siRNA expression. To identify highly efficacious siRNA sequences, ext...

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Detalles Bibliográficos
Autores principales:	Pang, Shen, Pokomo, Lauren, Chen, Kevin, Kamata, Masakazu, Mao, Si-Hua, Zhang, Hong, Razi, Elliot, An, Dong Sung, Chen, Irvin S. Y.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022502/ https://www.ncbi.nlm.nih.gov/pubmed/24831610 http://dx.doi.org/10.1371/journal.pone.0096445

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