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The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes

The chemokine receptor CXCR6 is expressed on different T cell subsets and up-regulated following T cell activation. CXCR6 has been implicated in the localization of cells to the liver due to the constitutive expression of its ligand CXCL16 on liver sinusoidal endothelial cells. Here, we analyzed the...

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Autores principales: Heesch, Kira, Raczkowski, Friederike, Schumacher, Valéa, Hünemörder, Stefanie, Panzer, Ulf, Mittrücker, Hans-Willi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022635/
https://www.ncbi.nlm.nih.gov/pubmed/24832098
http://dx.doi.org/10.1371/journal.pone.0097701
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author Heesch, Kira
Raczkowski, Friederike
Schumacher, Valéa
Hünemörder, Stefanie
Panzer, Ulf
Mittrücker, Hans-Willi
author_facet Heesch, Kira
Raczkowski, Friederike
Schumacher, Valéa
Hünemörder, Stefanie
Panzer, Ulf
Mittrücker, Hans-Willi
author_sort Heesch, Kira
collection PubMed
description The chemokine receptor CXCR6 is expressed on different T cell subsets and up-regulated following T cell activation. CXCR6 has been implicated in the localization of cells to the liver due to the constitutive expression of its ligand CXCL16 on liver sinusoidal endothelial cells. Here, we analyzed the role of CXCR6 in CD8(+) T cell responses to infection of mice with Listeria monocytogenes. CD8(+) T cells responding to listerial antigens acquired high expression levels of CXCR6. However, deficiency of mice in CXCR6 did not impair control of the L. monocytogenes infection. CXCR6-deficient mice were able to generate listeria-specific CD4(+) and CD8(+) T cell responses and showed accumulation of T cells in the infected liver. In transfer assays, we detected reduced accumulation of listeria-specific CXCR6-deficient CD8(+) T cells in the liver at early time points post infection. Though, CXCR6 was dispensable at later time points of the CD8(+) T cell response. When transferred CD8(+) T cells were followed for extended time periods, we observed a decline in CXCR6-deficient CD8(+) T cells. The manifestation of this cell loss depended on the tissue analyzed. In conclusion, our results demonstrate that CXCR6 is not required for the formation of a T cell response to L. monocytogenes and for the accumulation of T cells in the infected liver but CXCR6 appears to influence long-term survival and tissue distribution of activated cells.
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spelling pubmed-40226352014-05-21 The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes Heesch, Kira Raczkowski, Friederike Schumacher, Valéa Hünemörder, Stefanie Panzer, Ulf Mittrücker, Hans-Willi PLoS One Research Article The chemokine receptor CXCR6 is expressed on different T cell subsets and up-regulated following T cell activation. CXCR6 has been implicated in the localization of cells to the liver due to the constitutive expression of its ligand CXCL16 on liver sinusoidal endothelial cells. Here, we analyzed the role of CXCR6 in CD8(+) T cell responses to infection of mice with Listeria monocytogenes. CD8(+) T cells responding to listerial antigens acquired high expression levels of CXCR6. However, deficiency of mice in CXCR6 did not impair control of the L. monocytogenes infection. CXCR6-deficient mice were able to generate listeria-specific CD4(+) and CD8(+) T cell responses and showed accumulation of T cells in the infected liver. In transfer assays, we detected reduced accumulation of listeria-specific CXCR6-deficient CD8(+) T cells in the liver at early time points post infection. Though, CXCR6 was dispensable at later time points of the CD8(+) T cell response. When transferred CD8(+) T cells were followed for extended time periods, we observed a decline in CXCR6-deficient CD8(+) T cells. The manifestation of this cell loss depended on the tissue analyzed. In conclusion, our results demonstrate that CXCR6 is not required for the formation of a T cell response to L. monocytogenes and for the accumulation of T cells in the infected liver but CXCR6 appears to influence long-term survival and tissue distribution of activated cells. Public Library of Science 2014-05-15 /pmc/articles/PMC4022635/ /pubmed/24832098 http://dx.doi.org/10.1371/journal.pone.0097701 Text en © 2014 Heesch et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Heesch, Kira
Raczkowski, Friederike
Schumacher, Valéa
Hünemörder, Stefanie
Panzer, Ulf
Mittrücker, Hans-Willi
The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title_full The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title_fullStr The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title_full_unstemmed The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title_short The Function of the Chemokine Receptor CXCR6 in the T Cell Response of Mice against Listeria monocytogenes
title_sort function of the chemokine receptor cxcr6 in the t cell response of mice against listeria monocytogenes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022635/
https://www.ncbi.nlm.nih.gov/pubmed/24832098
http://dx.doi.org/10.1371/journal.pone.0097701
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