Cargando…

The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target

Plant microRNAs (miRNAs) are small, regulatory non-coding RNAs involved in a wide range of biological processes, from organ development to response to stimuli. In recent years, an increasing number of studies on model plant species have highlighted the evolutionary conservation of a high number of m...

Descripción completa

Detalles Bibliográficos
Autores principales: Aceto, Serena, Sica, Maria, De Paolo, Sofia, D'Argenio, Valeria, Cantiello, Piergiuseppe, Salvatore, Francesco, Gaudio, Luciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022656/
https://www.ncbi.nlm.nih.gov/pubmed/24832004
http://dx.doi.org/10.1371/journal.pone.0097839
_version_ 1782316447268077568
author Aceto, Serena
Sica, Maria
De Paolo, Sofia
D'Argenio, Valeria
Cantiello, Piergiuseppe
Salvatore, Francesco
Gaudio, Luciano
author_facet Aceto, Serena
Sica, Maria
De Paolo, Sofia
D'Argenio, Valeria
Cantiello, Piergiuseppe
Salvatore, Francesco
Gaudio, Luciano
author_sort Aceto, Serena
collection PubMed
description Plant microRNAs (miRNAs) are small, regulatory non-coding RNAs involved in a wide range of biological processes, from organ development to response to stimuli. In recent years, an increasing number of studies on model plant species have highlighted the evolutionary conservation of a high number of miRNA families and the existence of taxon-specific ones. However, few studies have examined miRNAs in non-model species such as orchids, which are characterized by highly diversified floral structures and pollination strategies. Therefore, we analysed a small RNA library of inflorescence tissue of the Mediterranean orchid Orchis italica to increase the knowledge on miRNAs in a non-model plant species. The high-throughput sequencing and analysis of a small RNA library of inflorescence of O. italica revealed 23 conserved and 161 putative novel miRNA families. Among the putative miRNA targets, experimental validation demonstrated that a DEF-like MADS-box transcript is cleaved by the homolog of miR5179 of O. italica. The presence of conserved miRNA families in the inflorescence of O. italica indicates that the basic developmental flower regulatory mechanisms mediated by miRNAs are maintained through evolution. Because, according to the “orchid code” theory, DEF-like genes exert a key function in the diversification of tepals and lip, the cleavage-mediated inhibitory activity of miR5179 on a OitaDEF-like transcript suggests that, in orchids, miRNAs play an important role in the diversification of the perianth organs.
format Online
Article
Text
id pubmed-4022656
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40226562014-05-21 The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target Aceto, Serena Sica, Maria De Paolo, Sofia D'Argenio, Valeria Cantiello, Piergiuseppe Salvatore, Francesco Gaudio, Luciano PLoS One Research Article Plant microRNAs (miRNAs) are small, regulatory non-coding RNAs involved in a wide range of biological processes, from organ development to response to stimuli. In recent years, an increasing number of studies on model plant species have highlighted the evolutionary conservation of a high number of miRNA families and the existence of taxon-specific ones. However, few studies have examined miRNAs in non-model species such as orchids, which are characterized by highly diversified floral structures and pollination strategies. Therefore, we analysed a small RNA library of inflorescence tissue of the Mediterranean orchid Orchis italica to increase the knowledge on miRNAs in a non-model plant species. The high-throughput sequencing and analysis of a small RNA library of inflorescence of O. italica revealed 23 conserved and 161 putative novel miRNA families. Among the putative miRNA targets, experimental validation demonstrated that a DEF-like MADS-box transcript is cleaved by the homolog of miR5179 of O. italica. The presence of conserved miRNA families in the inflorescence of O. italica indicates that the basic developmental flower regulatory mechanisms mediated by miRNAs are maintained through evolution. Because, according to the “orchid code” theory, DEF-like genes exert a key function in the diversification of tepals and lip, the cleavage-mediated inhibitory activity of miR5179 on a OitaDEF-like transcript suggests that, in orchids, miRNAs play an important role in the diversification of the perianth organs. Public Library of Science 2014-05-15 /pmc/articles/PMC4022656/ /pubmed/24832004 http://dx.doi.org/10.1371/journal.pone.0097839 Text en © 2014 Aceto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aceto, Serena
Sica, Maria
De Paolo, Sofia
D'Argenio, Valeria
Cantiello, Piergiuseppe
Salvatore, Francesco
Gaudio, Luciano
The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title_full The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title_fullStr The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title_full_unstemmed The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title_short The Analysis of the Inflorescence miRNome of the Orchid Orchis italica Reveals a DEF-Like MADS-Box Gene as a New miRNA Target
title_sort analysis of the inflorescence mirnome of the orchid orchis italica reveals a def-like mads-box gene as a new mirna target
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022656/
https://www.ncbi.nlm.nih.gov/pubmed/24832004
http://dx.doi.org/10.1371/journal.pone.0097839
work_keys_str_mv AT acetoserena theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT sicamaria theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT depaolosofia theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT dargeniovaleria theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT cantiellopiergiuseppe theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT salvatorefrancesco theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT gaudioluciano theanalysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT acetoserena analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT sicamaria analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT depaolosofia analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT dargeniovaleria analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT cantiellopiergiuseppe analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT salvatorefrancesco analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget
AT gaudioluciano analysisoftheinflorescencemirnomeoftheorchidorchisitalicarevealsadeflikemadsboxgeneasanewmirnatarget