A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus

PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion. The first stage of the restriction enzyme assay is hybridization of a target DNA to immob...

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Detalles Bibliográficos
Autores principales: Smith, Maria W., Ghindilis, Andrei L., Seoudi, Ihab A., Smith, Kenneth, Billharz, Rosalind, Simon, Holly M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022673/
https://www.ncbi.nlm.nih.gov/pubmed/24831802
http://dx.doi.org/10.1371/journal.pone.0097826

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