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Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis

BACKGROUND: The osteoarthritis (OA) treatment in humans and in animals is a major orthopaedic challenge because there is not an ideal drug for preserving the joint structure and function. The aim of this study was to assess the effects of the treatment with oral glucosamine and risedronate alone or...

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Autores principales: Permuy, María, Guede, David, López-Peña, Mónica, Muñoz, Fernando, González-Cantalapiedra, Antonio, Caeiro, Jose-Ramón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4023551/
https://www.ncbi.nlm.nih.gov/pubmed/24766775
http://dx.doi.org/10.1186/1746-6148-10-97
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author Permuy, María
Guede, David
López-Peña, Mónica
Muñoz, Fernando
González-Cantalapiedra, Antonio
Caeiro, Jose-Ramón
author_facet Permuy, María
Guede, David
López-Peña, Mónica
Muñoz, Fernando
González-Cantalapiedra, Antonio
Caeiro, Jose-Ramón
author_sort Permuy, María
collection PubMed
description BACKGROUND: The osteoarthritis (OA) treatment in humans and in animals is a major orthopaedic challenge because there is not an ideal drug for preserving the joint structure and function. The aim of this study was to assess the effects of the treatment with oral glucosamine and risedronate alone or in combination on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of OA. Osteoarthritis was surgically induced on one knee of 32 New Zealand White rabbits using the contralateral as healthy controls. Three weeks later treatments were started and lasted 8 weeks. Animal were divided in four groups of oral treatment: the first group received only saline, the second 21.5 mg/kg/day of glucosamine sulfate, the third 0.07 mg/kg/day of risedronate; and the fourth group both drugs simultaneously at the same dosages. Following sacrifice femurs were removed and osteochondral cylinders and synovial membrane were obtained for its histological and micro-CT evaluation. RESULTS: Sample analysis revealed that the model induced osteoarthritic changes in operated knees. OA placebo group showed a significant increase in cartilage thickness respect to the control and inflammatory changes in synovial membrane; whereas subchondral bone structure and volumetric bone mineral density remained unchanged. All the treated animals showed an improvement of the cartilage swelling independent of the drug used. Treatment with glucosamine alone seemed to have no effect in the progression of cartilage pathology while risedronate treatment had better results in superficial fibrillation and in resolving the inflammatory changes of the tissues, as well as modifying the orientation of trabecular lattice. The combination of both compounds seemed to have additive effects showing better results than those treated with only one drug. CONCLUSIONS: The results of this animal study suggested that glucosamine sulfate and risedronate treatment alone or in combination may be able to stop cartilage swelling. The risedronate treatment could partially stop the fibrillation and the inflammation of synovial membrane as well as modify the orientation of trabeculae in healthy and in osteoarthritic knees.
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spelling pubmed-40235512014-05-17 Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis Permuy, María Guede, David López-Peña, Mónica Muñoz, Fernando González-Cantalapiedra, Antonio Caeiro, Jose-Ramón BMC Vet Res Research Article BACKGROUND: The osteoarthritis (OA) treatment in humans and in animals is a major orthopaedic challenge because there is not an ideal drug for preserving the joint structure and function. The aim of this study was to assess the effects of the treatment with oral glucosamine and risedronate alone or in combination on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of OA. Osteoarthritis was surgically induced on one knee of 32 New Zealand White rabbits using the contralateral as healthy controls. Three weeks later treatments were started and lasted 8 weeks. Animal were divided in four groups of oral treatment: the first group received only saline, the second 21.5 mg/kg/day of glucosamine sulfate, the third 0.07 mg/kg/day of risedronate; and the fourth group both drugs simultaneously at the same dosages. Following sacrifice femurs were removed and osteochondral cylinders and synovial membrane were obtained for its histological and micro-CT evaluation. RESULTS: Sample analysis revealed that the model induced osteoarthritic changes in operated knees. OA placebo group showed a significant increase in cartilage thickness respect to the control and inflammatory changes in synovial membrane; whereas subchondral bone structure and volumetric bone mineral density remained unchanged. All the treated animals showed an improvement of the cartilage swelling independent of the drug used. Treatment with glucosamine alone seemed to have no effect in the progression of cartilage pathology while risedronate treatment had better results in superficial fibrillation and in resolving the inflammatory changes of the tissues, as well as modifying the orientation of trabecular lattice. The combination of both compounds seemed to have additive effects showing better results than those treated with only one drug. CONCLUSIONS: The results of this animal study suggested that glucosamine sulfate and risedronate treatment alone or in combination may be able to stop cartilage swelling. The risedronate treatment could partially stop the fibrillation and the inflammation of synovial membrane as well as modify the orientation of trabeculae in healthy and in osteoarthritic knees. BioMed Central 2014-04-26 /pmc/articles/PMC4023551/ /pubmed/24766775 http://dx.doi.org/10.1186/1746-6148-10-97 Text en Copyright © 2014 Permuy et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Permuy, María
Guede, David
López-Peña, Mónica
Muñoz, Fernando
González-Cantalapiedra, Antonio
Caeiro, Jose-Ramón
Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title_full Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title_fullStr Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title_full_unstemmed Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title_short Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
title_sort effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4023551/
https://www.ncbi.nlm.nih.gov/pubmed/24766775
http://dx.doi.org/10.1186/1746-6148-10-97
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