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MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis

We previously reported frequent loss of microRNA-218 (miR-218) in cervical cancer, which was associated with tumor progression and poor prognosis. As microRNAs were found invovled in the regulation of radiosensitivity in various human cancers, we therefore aim to investigate the effects of miR-218 o...

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Autores principales: Yuan, Wang, Xiaoyun, Han, Haifeng, Qiu, Jing, Li, Weixu, Hu, Ruofan, Dong, Jinjin, Yu, Zongji, Shen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025168/
https://www.ncbi.nlm.nih.gov/pubmed/24843318
http://dx.doi.org/10.7150/ijms.8880
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author Yuan, Wang
Xiaoyun, Han
Haifeng, Qiu
Jing, Li
Weixu, Hu
Ruofan, Dong
Jinjin, Yu
Zongji, Shen
author_facet Yuan, Wang
Xiaoyun, Han
Haifeng, Qiu
Jing, Li
Weixu, Hu
Ruofan, Dong
Jinjin, Yu
Zongji, Shen
author_sort Yuan, Wang
collection PubMed
description We previously reported frequent loss of microRNA-218 (miR-218) in cervical cancer, which was associated with tumor progression and poor prognosis. As microRNAs were found invovled in the regulation of radiosensitivity in various human cancers, we therefore aim to investigate the effects of miR-218 on radiosensitivity of cervical cancer in the present study. The clonogenic survival assay demonstrated that loss of miR-218 could predict radioresistance in the primary cervical cancer cells (R(2)=0.6516, P<0.001). In vitro, abundant miR-218 increased the radiosensitivity in cervical cancer cells (P<0.001 for HeLa, P=0.009 for SiHa, P=0.016 for C33A and P=0.01 for CaSki). Upregulation of miR-218 significantly enhanced the radiation-induced apoptosis, which was further enhanced by the combination of miR-218 overexpression and radiation In xenograft growth assay, combination of miR-218 overexpression and radiation notably induced cellular apoptosis and suppressed tumor growth. In conclusion, we demonstrated that miR-218 resensitized cervical cancer cells to radiation via promoting cellular apoptosis. Moreover, we proved that miR-218 as a potent predictor of radiosensitivity in cervical cancer, especially for those patients with loss of miR-218.
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spelling pubmed-40251682014-05-19 MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis Yuan, Wang Xiaoyun, Han Haifeng, Qiu Jing, Li Weixu, Hu Ruofan, Dong Jinjin, Yu Zongji, Shen Int J Med Sci Research Paper We previously reported frequent loss of microRNA-218 (miR-218) in cervical cancer, which was associated with tumor progression and poor prognosis. As microRNAs were found invovled in the regulation of radiosensitivity in various human cancers, we therefore aim to investigate the effects of miR-218 on radiosensitivity of cervical cancer in the present study. The clonogenic survival assay demonstrated that loss of miR-218 could predict radioresistance in the primary cervical cancer cells (R(2)=0.6516, P<0.001). In vitro, abundant miR-218 increased the radiosensitivity in cervical cancer cells (P<0.001 for HeLa, P=0.009 for SiHa, P=0.016 for C33A and P=0.01 for CaSki). Upregulation of miR-218 significantly enhanced the radiation-induced apoptosis, which was further enhanced by the combination of miR-218 overexpression and radiation In xenograft growth assay, combination of miR-218 overexpression and radiation notably induced cellular apoptosis and suppressed tumor growth. In conclusion, we demonstrated that miR-218 resensitized cervical cancer cells to radiation via promoting cellular apoptosis. Moreover, we proved that miR-218 as a potent predictor of radiosensitivity in cervical cancer, especially for those patients with loss of miR-218. Ivyspring International Publisher 2014-05-06 /pmc/articles/PMC4025168/ /pubmed/24843318 http://dx.doi.org/10.7150/ijms.8880 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Yuan, Wang
Xiaoyun, Han
Haifeng, Qiu
Jing, Li
Weixu, Hu
Ruofan, Dong
Jinjin, Yu
Zongji, Shen
MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title_full MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title_fullStr MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title_full_unstemmed MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title_short MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis
title_sort microrna-218 enhances the radiosensitivity of human cervical cancer via promoting radiation induced apoptosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025168/
https://www.ncbi.nlm.nih.gov/pubmed/24843318
http://dx.doi.org/10.7150/ijms.8880
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