Efficient Gene Knock-out and Knock-in with Transgenic Cas9 in Drosophila

Bacterial Cas9 nuclease induces site-specific DNA breaks using small gRNA as guides. Cas9 has been successfully introduced into Drosophila for genome editing. Here, we improve the versatility of this method by developing a transgenic system that expresses Cas9 in the Drosophila germline. Using this...

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Detalles Bibliográficos
Autores principales: Xue, Zhaoyu, Ren, Mengda, Wu, Menghua, Dai, Junbiao, Rong, Yikang S., Gao, Guanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025491/
https://www.ncbi.nlm.nih.gov/pubmed/24657904
http://dx.doi.org/10.1534/g3.114.010496

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025491/
https://www.ncbi.nlm.nih.gov/pubmed/24657904
http://dx.doi.org/10.1534/g3.114.010496

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