Cargando…
Efficient Gene Knock-out and Knock-in with Transgenic Cas9 in Drosophila
Bacterial Cas9 nuclease induces site-specific DNA breaks using small gRNA as guides. Cas9 has been successfully introduced into Drosophila for genome editing. Here, we improve the versatility of this method by developing a transgenic system that expresses Cas9 in the Drosophila germline. Using this...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025491/ https://www.ncbi.nlm.nih.gov/pubmed/24657904 http://dx.doi.org/10.1534/g3.114.010496 |