Cargando…
Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin
Many experiments conducted in the literature have investigated the effect of microneedles (MNs) on insulin permeation across skin. There are also a number of articles that deal with the effect of MN insertion force in skin. However, there is little known on quantifying the relationship between the e...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025618/ https://www.ncbi.nlm.nih.gov/pubmed/24876604 http://dx.doi.org/10.1177/1932296813519720 |
_version_ | 1782316794695909376 |
---|---|
author | Cheung, Karmen Han, Tao Das, Diganta Bhusan |
author_facet | Cheung, Karmen Han, Tao Das, Diganta Bhusan |
author_sort | Cheung, Karmen |
collection | PubMed |
description | Many experiments conducted in the literature have investigated the effect of microneedles (MNs) on insulin permeation across skin. There are also a number of articles that deal with the effect of MN insertion force in skin. However, there is little known on quantifying the relationship between the effect of MN insertion force and the amount of insulin permeated for given MNs. This issue is addressed in this article. MNs of 1100 µm and 1400 µm are used to conduct in vitro permeability experiments on porcine skin, using insulin. Histological images of MN treated skin are obtained from a microtome and the viscoelastic properties of the skin sample are measured using a rheometer. An in-house insertion force device is utilized that can reproducibly apply a specified force on MNs for a set period of time using compressed air. It is deduced that when porcine skin was pretreated with an applied force of 60.5 N and 69.1 N, the resultant amount of insulin permeated was approximately 3 µg and 25 µg over a 4-hour period for the MNs used. The amount of MN force applied to porcine skin was shown to be related to the amount of insulin permeated. An increase in insertion force increase the amount of insulin permeated. It was also demonstrated that using insufficient force may have reduced or prevented the amount of insulin passing through the skin, regardless of the geometry of the MNs. |
format | Online Article Text |
id | pubmed-4025618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-40256182014-05-20 Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin Cheung, Karmen Han, Tao Das, Diganta Bhusan J Diabetes Sci Technol Special Section: Novel Methods for Delivering Insulin Many experiments conducted in the literature have investigated the effect of microneedles (MNs) on insulin permeation across skin. There are also a number of articles that deal with the effect of MN insertion force in skin. However, there is little known on quantifying the relationship between the effect of MN insertion force and the amount of insulin permeated for given MNs. This issue is addressed in this article. MNs of 1100 µm and 1400 µm are used to conduct in vitro permeability experiments on porcine skin, using insulin. Histological images of MN treated skin are obtained from a microtome and the viscoelastic properties of the skin sample are measured using a rheometer. An in-house insertion force device is utilized that can reproducibly apply a specified force on MNs for a set period of time using compressed air. It is deduced that when porcine skin was pretreated with an applied force of 60.5 N and 69.1 N, the resultant amount of insulin permeated was approximately 3 µg and 25 µg over a 4-hour period for the MNs used. The amount of MN force applied to porcine skin was shown to be related to the amount of insulin permeated. An increase in insertion force increase the amount of insulin permeated. It was also demonstrated that using insufficient force may have reduced or prevented the amount of insulin passing through the skin, regardless of the geometry of the MNs. SAGE Publications 2014-05 /pmc/articles/PMC4025618/ /pubmed/24876604 http://dx.doi.org/10.1177/1932296813519720 Text en © 2014 Diabetes Technology Society http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm). |
spellingShingle | Special Section: Novel Methods for Delivering Insulin Cheung, Karmen Han, Tao Das, Diganta Bhusan Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title | Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title_full | Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title_fullStr | Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title_full_unstemmed | Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title_short | Effect of Force of Microneedle Insertion on the Permeability of Insulin in Skin |
title_sort | effect of force of microneedle insertion on the permeability of insulin in skin |
topic | Special Section: Novel Methods for Delivering Insulin |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025618/ https://www.ncbi.nlm.nih.gov/pubmed/24876604 http://dx.doi.org/10.1177/1932296813519720 |
work_keys_str_mv | AT cheungkarmen effectofforceofmicroneedleinsertiononthepermeabilityofinsulininskin AT hantao effectofforceofmicroneedleinsertiononthepermeabilityofinsulininskin AT dasdigantabhusan effectofforceofmicroneedleinsertiononthepermeabilityofinsulininskin |