A synthetic luciferin improves bioluminescence imaging in live mice

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice wi...

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Detalles Bibliográficos
Autores principales: Evans, Melanie S., Chaurette, Joanna P., Adams, Spencer T., Reddy, Gadarla R., Paley, Miranda A., Aronin, Neil, Prescher, Jennifer A., Miller, Stephen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026177/
https://www.ncbi.nlm.nih.gov/pubmed/24509630
http://dx.doi.org/10.1038/nmeth.2839
Descripción
Sumario:Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI from existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.

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