A synthetic luciferin improves bioluminescence imaging in live mice

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice wi...

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Autores principales: Evans, Melanie S., Chaurette, Joanna P., Adams, Spencer T., Reddy, Gadarla R., Paley, Miranda A., Aronin, Neil, Prescher, Jennifer A., Miller, Stephen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026177/
https://www.ncbi.nlm.nih.gov/pubmed/24509630
http://dx.doi.org/10.1038/nmeth.2839
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author Evans, Melanie S.
Chaurette, Joanna P.
Adams, Spencer T.
Reddy, Gadarla R.
Paley, Miranda A.
Aronin, Neil
Prescher, Jennifer A.
Miller, Stephen C.
author_facet Evans, Melanie S.
Chaurette, Joanna P.
Adams, Spencer T.
Reddy, Gadarla R.
Paley, Miranda A.
Aronin, Neil
Prescher, Jennifer A.
Miller, Stephen C.
author_sort Evans, Melanie S.
collection PubMed
description Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI from existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
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spelling pubmed-40261772014-10-01 A synthetic luciferin improves bioluminescence imaging in live mice Evans, Melanie S. Chaurette, Joanna P. Adams, Spencer T. Reddy, Gadarla R. Paley, Miranda A. Aronin, Neil Prescher, Jennifer A. Miller, Stephen C. Nat Methods Article Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI from existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin. 2014-02-09 2014-04 /pmc/articles/PMC4026177/ /pubmed/24509630 http://dx.doi.org/10.1038/nmeth.2839 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Evans, Melanie S.
Chaurette, Joanna P.
Adams, Spencer T.
Reddy, Gadarla R.
Paley, Miranda A.
Aronin, Neil
Prescher, Jennifer A.
Miller, Stephen C.
A synthetic luciferin improves bioluminescence imaging in live mice
title A synthetic luciferin improves bioluminescence imaging in live mice
title_full A synthetic luciferin improves bioluminescence imaging in live mice
title_fullStr A synthetic luciferin improves bioluminescence imaging in live mice
title_full_unstemmed A synthetic luciferin improves bioluminescence imaging in live mice
title_short A synthetic luciferin improves bioluminescence imaging in live mice
title_sort synthetic luciferin improves bioluminescence imaging in live mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026177/
https://www.ncbi.nlm.nih.gov/pubmed/24509630
http://dx.doi.org/10.1038/nmeth.2839
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