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Snapback Primer Mediated Clamping PCR for Detection of EGFR and KRAS Mutations in NSCLC Patients by High Resolution Melting Analysis

Assays for detecting somatic mutations are requested with higher sensitivity and more convenience. Here, we describe snapback primer mediated allele clamping enrichment polymerase chain reaction (SPACE-PCR), a novel form of PCR that amplifies minority alleles selectively from mixtures. We replaced r...

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Detalles Bibliográficos
Autores principales:	Sun, Haiyan, Yang, Yang, Yang, Lixin, Su, Bo, Jiang, Gening, Fei, Ke, Lu, Daru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026848/ https://www.ncbi.nlm.nih.gov/pubmed/24883309 http://dx.doi.org/10.1155/2014/407537

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