Pervasive generation of oppositely oriented spacers during CRISPR adaptation

During the process of prokaryotic CRISPR adaptation, a copy of a segment of foreign deoxyribonucleic acid referred to as protospacer is added to the CRISPR cassette and becomes a spacer. When a protospacer contains a neighboring target interference motif, the specific small CRISPR ribonucleic acid (...

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Detalles Bibliográficos
Autores principales: Shmakov, Sergey, Savitskaya, Ekaterina, Semenova, Ekaterina, Logacheva, Maria D., Datsenko, Kirill A., Severinov, Konstantin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027179/
https://www.ncbi.nlm.nih.gov/pubmed/24728991
http://dx.doi.org/10.1093/nar/gku226

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