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The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers
Recent studies have shown that cancer immunotherapy could be a promising therapeutic approach for the treatment of cancer. In the present study, to identify novel tumor-associated antigens (TAAs), the proteins expressed in a panel of cancer cells were serologically screened by immunoblot analysis an...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027928/ https://www.ncbi.nlm.nih.gov/pubmed/24589652 http://dx.doi.org/10.3892/ijo.2014.2318 |
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author | OJI, YUSUKE TATSUMI, NAOYA FUKUDA, MARI NAKATSUKA, SHIN-ICHI AOYAGI, SAYAKA HIRATA, ERIKA NANCHI, ISAMU FUJIKI, FUMIHIRO NAKAJIMA, HIROKO YAMAMOTO, YUMIKO SHIBATA, SYOHEI NAKAMURA, MICHIYO HASEGAWA, KANA TAKAGI, SAYAKA FUKUDA, IKUYO HOSHIKAWA, TOMOKO MURAKAMI, YUI MORI, MASAHIDE INOUE, MASAYOSHI NAKA, TETSUJI TOMONAGA, TAKESHI SHIMIZU, YOSHIFUMI NAKAGAWA, MASASHI HASEGAWA, JUNICHI NEZU, RIICHIRO INOHARA, HIDENORI IZUMOTO, SHUICHI NONOMURA, NORIO YOSHIMINE, TOSHIKI OKUMURA, MEINOSHIN MORII, EIICHI MAEDA, HAJIME NISHIDA, SUMIYUKI HOSEN, NAOKI TSUBOI, AKIHIRO OKA, YOSHIHIRO SUGIYAMA, HARUO |
author_facet | OJI, YUSUKE TATSUMI, NAOYA FUKUDA, MARI NAKATSUKA, SHIN-ICHI AOYAGI, SAYAKA HIRATA, ERIKA NANCHI, ISAMU FUJIKI, FUMIHIRO NAKAJIMA, HIROKO YAMAMOTO, YUMIKO SHIBATA, SYOHEI NAKAMURA, MICHIYO HASEGAWA, KANA TAKAGI, SAYAKA FUKUDA, IKUYO HOSHIKAWA, TOMOKO MURAKAMI, YUI MORI, MASAHIDE INOUE, MASAYOSHI NAKA, TETSUJI TOMONAGA, TAKESHI SHIMIZU, YOSHIFUMI NAKAGAWA, MASASHI HASEGAWA, JUNICHI NEZU, RIICHIRO INOHARA, HIDENORI IZUMOTO, SHUICHI NONOMURA, NORIO YOSHIMINE, TOSHIKI OKUMURA, MEINOSHIN MORII, EIICHI MAEDA, HAJIME NISHIDA, SUMIYUKI HOSEN, NAOKI TSUBOI, AKIHIRO OKA, YOSHIHIRO SUGIYAMA, HARUO |
author_sort | OJI, YUSUKE |
collection | PubMed |
description | Recent studies have shown that cancer immunotherapy could be a promising therapeutic approach for the treatment of cancer. In the present study, to identify novel tumor-associated antigens (TAAs), the proteins expressed in a panel of cancer cells were serologically screened by immunoblot analysis and the eukaryotic elongation factor 2 (eEF2) was identified as an antigen that was recognized by IgG autoantibody in sera from a group of patients with head and neck squamous cell carcinoma (HNSCC) or colon cancer. Enzyme-linked immunosorbent assay showed that serum eEF2 IgG Ab levels were significantly higher in colorectal and gastric cancer patients compared to healthy individuals. Immunohistochemistry experiments showed that the eEF2 protein was overexpressed in the majority of lung, esophageal, pancreatic, breast and prostate cancers, HNSCC, glioblastoma multiforme and non-Hodgkin’s lymphoma (NHL). Knockdown of eEF2 by short hairpin RNA (shRNA) significantly inhibited the growth in four eEF2-expressing cell lines, PC14 lung cancer, PCI6 pancreatic cancer, HT1080 fibrosarcoma and A172 glioblastoma cells, but not in eEF2-undetectable MCF7 cells. Furthermore, eEF2-derived 9-mer peptides, EF786 (eEF2 786–794 aa) and EF292 (eEF2 292–300 aa), elicited cytotoxic T lymphocyte (CTL) responses in peripheral blood mononuclear cells (PBMCs) from an HLA-A(*)24:02- and an HLA-A(*)02:01-positive healthy donor, respectively, in an HLA-A-restricted manner. These results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth. Moreover, the eEF2 gene product is immunogenic and a promising target molecule of cancer immunotherapy for several types of cancers. |
format | Online Article Text |
id | pubmed-4027928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-40279282014-05-20 The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers OJI, YUSUKE TATSUMI, NAOYA FUKUDA, MARI NAKATSUKA, SHIN-ICHI AOYAGI, SAYAKA HIRATA, ERIKA NANCHI, ISAMU FUJIKI, FUMIHIRO NAKAJIMA, HIROKO YAMAMOTO, YUMIKO SHIBATA, SYOHEI NAKAMURA, MICHIYO HASEGAWA, KANA TAKAGI, SAYAKA FUKUDA, IKUYO HOSHIKAWA, TOMOKO MURAKAMI, YUI MORI, MASAHIDE INOUE, MASAYOSHI NAKA, TETSUJI TOMONAGA, TAKESHI SHIMIZU, YOSHIFUMI NAKAGAWA, MASASHI HASEGAWA, JUNICHI NEZU, RIICHIRO INOHARA, HIDENORI IZUMOTO, SHUICHI NONOMURA, NORIO YOSHIMINE, TOSHIKI OKUMURA, MEINOSHIN MORII, EIICHI MAEDA, HAJIME NISHIDA, SUMIYUKI HOSEN, NAOKI TSUBOI, AKIHIRO OKA, YOSHIHIRO SUGIYAMA, HARUO Int J Oncol Article Recent studies have shown that cancer immunotherapy could be a promising therapeutic approach for the treatment of cancer. In the present study, to identify novel tumor-associated antigens (TAAs), the proteins expressed in a panel of cancer cells were serologically screened by immunoblot analysis and the eukaryotic elongation factor 2 (eEF2) was identified as an antigen that was recognized by IgG autoantibody in sera from a group of patients with head and neck squamous cell carcinoma (HNSCC) or colon cancer. Enzyme-linked immunosorbent assay showed that serum eEF2 IgG Ab levels were significantly higher in colorectal and gastric cancer patients compared to healthy individuals. Immunohistochemistry experiments showed that the eEF2 protein was overexpressed in the majority of lung, esophageal, pancreatic, breast and prostate cancers, HNSCC, glioblastoma multiforme and non-Hodgkin’s lymphoma (NHL). Knockdown of eEF2 by short hairpin RNA (shRNA) significantly inhibited the growth in four eEF2-expressing cell lines, PC14 lung cancer, PCI6 pancreatic cancer, HT1080 fibrosarcoma and A172 glioblastoma cells, but not in eEF2-undetectable MCF7 cells. Furthermore, eEF2-derived 9-mer peptides, EF786 (eEF2 786–794 aa) and EF292 (eEF2 292–300 aa), elicited cytotoxic T lymphocyte (CTL) responses in peripheral blood mononuclear cells (PBMCs) from an HLA-A(*)24:02- and an HLA-A(*)02:01-positive healthy donor, respectively, in an HLA-A-restricted manner. These results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth. Moreover, the eEF2 gene product is immunogenic and a promising target molecule of cancer immunotherapy for several types of cancers. D.A. Spandidos 2014-03-04 /pmc/articles/PMC4027928/ /pubmed/24589652 http://dx.doi.org/10.3892/ijo.2014.2318 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Article OJI, YUSUKE TATSUMI, NAOYA FUKUDA, MARI NAKATSUKA, SHIN-ICHI AOYAGI, SAYAKA HIRATA, ERIKA NANCHI, ISAMU FUJIKI, FUMIHIRO NAKAJIMA, HIROKO YAMAMOTO, YUMIKO SHIBATA, SYOHEI NAKAMURA, MICHIYO HASEGAWA, KANA TAKAGI, SAYAKA FUKUDA, IKUYO HOSHIKAWA, TOMOKO MURAKAMI, YUI MORI, MASAHIDE INOUE, MASAYOSHI NAKA, TETSUJI TOMONAGA, TAKESHI SHIMIZU, YOSHIFUMI NAKAGAWA, MASASHI HASEGAWA, JUNICHI NEZU, RIICHIRO INOHARA, HIDENORI IZUMOTO, SHUICHI NONOMURA, NORIO YOSHIMINE, TOSHIKI OKUMURA, MEINOSHIN MORII, EIICHI MAEDA, HAJIME NISHIDA, SUMIYUKI HOSEN, NAOKI TSUBOI, AKIHIRO OKA, YOSHIHIRO SUGIYAMA, HARUO The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title | The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title_full | The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title_fullStr | The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title_full_unstemmed | The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title_short | The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers |
title_sort | translation elongation factor eef2 is a novel tumor-associated antigen overexpressed in various types of cancers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027928/ https://www.ncbi.nlm.nih.gov/pubmed/24589652 http://dx.doi.org/10.3892/ijo.2014.2318 |
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