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Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats

BACKGROUND: Since ancient times, honey has been used for medicinal purposes in many cultures; it is one of the oldest and most enduring substances used in wound management. Scientific evidence for its efficacy is widely studied, but systemic safety studies are still lacking. It is essential to study...

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Autores principales: Samat, Suhana, Nor, Nor Azmi Md, Nor Hussein, Fuzina, Ismail, Wan Iryani Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028288/
https://www.ncbi.nlm.nih.gov/pubmed/24885010
http://dx.doi.org/10.1186/1472-6882-14-146
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author Samat, Suhana
Nor, Nor Azmi Md
Nor Hussein, Fuzina
Ismail, Wan Iryani Wan
author_facet Samat, Suhana
Nor, Nor Azmi Md
Nor Hussein, Fuzina
Ismail, Wan Iryani Wan
author_sort Samat, Suhana
collection PubMed
description BACKGROUND: Since ancient times, honey has been used for medicinal purposes in many cultures; it is one of the oldest and most enduring substances used in wound management. Scientific evidence for its efficacy is widely studied, but systemic safety studies are still lacking. It is essential to study the impact of consumption of honey on the health and proper development of the consumer. Therefore, the present study was designed to observe the effects of acute administration (14 days) of Gelam honey (GH), a wild harvesting honey and Acacia honey (AH), a beekeeping honey, on male and female Sprague Dawley (SD) rats. METHODS: An acute oral study was performed following OECD test guideline 423, with minor modifications. In the study, GH, AH and sucrose (S) were administered at 2000 mg/kg body weight. Animals were observed for the next 14 days. Gross pathology was performed at the end of the study. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Clinical biochemistry, gross pathology, relative organ weight and histopathological examination were performed. RESULTS: Rats fed with honey did not exhibit any abnormal signs or deaths. Results showed a decrease in weight gain and energy efficiency, but significantly increased in total food intake and total calories in female rats fed with GH, compared to control (p < 0.05). Nevertheless, a significant increase in body weight was observed in male rats in all honey-treated groups. Male rats fed with AH significantly decreased in total food intake, total calories and energy efficiency. Both male and female rats fed with GH displayed a significant decrease in triglycerides compared to control group. Hepatic and renal function levels were within acceptable range. The gross necropsy analysis did not reveal changes in any of the organs examined. CONCLUSIONS: Our results suggest that acute consumption of GH and AH at 2000 mg/kg body weight of male and female SD rats has some discrepancy effects on biochemical parameters but in line with OECD regulation. Gelam honey may have potential in controlling weight gain and triglyceride levels in female rats compared to Acacia honey. SD rats have some effect on biochemical parameters, an exploration of which would make for intriguing analysis.
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spelling pubmed-40282882014-05-30 Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats Samat, Suhana Nor, Nor Azmi Md Nor Hussein, Fuzina Ismail, Wan Iryani Wan BMC Complement Altern Med Research Article BACKGROUND: Since ancient times, honey has been used for medicinal purposes in many cultures; it is one of the oldest and most enduring substances used in wound management. Scientific evidence for its efficacy is widely studied, but systemic safety studies are still lacking. It is essential to study the impact of consumption of honey on the health and proper development of the consumer. Therefore, the present study was designed to observe the effects of acute administration (14 days) of Gelam honey (GH), a wild harvesting honey and Acacia honey (AH), a beekeeping honey, on male and female Sprague Dawley (SD) rats. METHODS: An acute oral study was performed following OECD test guideline 423, with minor modifications. In the study, GH, AH and sucrose (S) were administered at 2000 mg/kg body weight. Animals were observed for the next 14 days. Gross pathology was performed at the end of the study. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Clinical biochemistry, gross pathology, relative organ weight and histopathological examination were performed. RESULTS: Rats fed with honey did not exhibit any abnormal signs or deaths. Results showed a decrease in weight gain and energy efficiency, but significantly increased in total food intake and total calories in female rats fed with GH, compared to control (p < 0.05). Nevertheless, a significant increase in body weight was observed in male rats in all honey-treated groups. Male rats fed with AH significantly decreased in total food intake, total calories and energy efficiency. Both male and female rats fed with GH displayed a significant decrease in triglycerides compared to control group. Hepatic and renal function levels were within acceptable range. The gross necropsy analysis did not reveal changes in any of the organs examined. CONCLUSIONS: Our results suggest that acute consumption of GH and AH at 2000 mg/kg body weight of male and female SD rats has some discrepancy effects on biochemical parameters but in line with OECD regulation. Gelam honey may have potential in controlling weight gain and triglyceride levels in female rats compared to Acacia honey. SD rats have some effect on biochemical parameters, an exploration of which would make for intriguing analysis. BioMed Central 2014-05-04 /pmc/articles/PMC4028288/ /pubmed/24885010 http://dx.doi.org/10.1186/1472-6882-14-146 Text en Copyright © 2014 Samat et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Samat, Suhana
Nor, Nor Azmi Md
Nor Hussein, Fuzina
Ismail, Wan Iryani Wan
Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title_full Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title_fullStr Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title_full_unstemmed Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title_short Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats
title_sort effects of gelam and acacia honey acute administration on some biochemical parameters of sprague dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028288/
https://www.ncbi.nlm.nih.gov/pubmed/24885010
http://dx.doi.org/10.1186/1472-6882-14-146
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