Cargando…

Nuclear assembly shaped by microtubule dynamics

Maintenance of nuclear architecture is crucial for gene regulation, cell proliferation and tissue development. However, during every open mitosis and meiosis, chromosomes are exposed to cytoskeletal forces until they are fully reassembled into mature nuclei. Here we discuss our recent study of nucle...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, John Z, Funabiki, Hironori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028354/
https://www.ncbi.nlm.nih.gov/pubmed/24637392
http://dx.doi.org/10.4161/nucl.28168
_version_ 1782317068956205056
author Xue, John Z
Funabiki, Hironori
author_facet Xue, John Z
Funabiki, Hironori
author_sort Xue, John Z
collection PubMed
description Maintenance of nuclear architecture is crucial for gene regulation, cell proliferation and tissue development. However, during every open mitosis and meiosis, chromosomes are exposed to cytoskeletal forces until they are fully reassembled into mature nuclei. Here we discuss our recent study of nuclear assembly in Xenopus egg extracts, where we showed that the DNA binding protein Developmental pluripotency associated 2 (Dppa2) directly inhibits microtubule polymerization during nuclear formation, and that this is essential for normal nuclear shape and replication. We explore mechanisms by which microtubule dynamics could regulate nuclear formation and morphology, and discuss the importance of both spatial and temporal regulation of microtubules in this process. Moreover, expression of Dppa2 is limited to the early embryo and pluripotent tissues, and we highlight the specific demands of mitosis in these often rapidly dividing cells, in which telophase nuclear assembly must be expedited and may facilitate developmental changes in nuclear architecture.
format Online
Article
Text
id pubmed-4028354
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Landes Bioscience
record_format MEDLINE/PubMed
spelling pubmed-40283542015-01-01 Nuclear assembly shaped by microtubule dynamics Xue, John Z Funabiki, Hironori Nucleus Extra View Maintenance of nuclear architecture is crucial for gene regulation, cell proliferation and tissue development. However, during every open mitosis and meiosis, chromosomes are exposed to cytoskeletal forces until they are fully reassembled into mature nuclei. Here we discuss our recent study of nuclear assembly in Xenopus egg extracts, where we showed that the DNA binding protein Developmental pluripotency associated 2 (Dppa2) directly inhibits microtubule polymerization during nuclear formation, and that this is essential for normal nuclear shape and replication. We explore mechanisms by which microtubule dynamics could regulate nuclear formation and morphology, and discuss the importance of both spatial and temporal regulation of microtubules in this process. Moreover, expression of Dppa2 is limited to the early embryo and pluripotent tissues, and we highlight the specific demands of mitosis in these often rapidly dividing cells, in which telophase nuclear assembly must be expedited and may facilitate developmental changes in nuclear architecture. Landes Bioscience 2014-01-01 2014-02-10 /pmc/articles/PMC4028354/ /pubmed/24637392 http://dx.doi.org/10.4161/nucl.28168 Text en Copyright © 2014 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Extra View
Xue, John Z
Funabiki, Hironori
Nuclear assembly shaped by microtubule dynamics
title Nuclear assembly shaped by microtubule dynamics
title_full Nuclear assembly shaped by microtubule dynamics
title_fullStr Nuclear assembly shaped by microtubule dynamics
title_full_unstemmed Nuclear assembly shaped by microtubule dynamics
title_short Nuclear assembly shaped by microtubule dynamics
title_sort nuclear assembly shaped by microtubule dynamics
topic Extra View
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028354/
https://www.ncbi.nlm.nih.gov/pubmed/24637392
http://dx.doi.org/10.4161/nucl.28168
work_keys_str_mv AT xuejohnz nuclearassemblyshapedbymicrotubuledynamics
AT funabikihironori nuclearassemblyshapedbymicrotubuledynamics