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A mitotic spindle-independent cleavage furrow positioning pathway

The mitotic spindle determines the cleavage furrow site during metazoan cell division(1,2), but whether other mechanisms exist remains unknown. Here we identify a spindle-independent mechanism for cleavage furrow positioning in Drosophila neuroblasts. We show that early and late furrow proteins (Pav...

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Detalles Bibliográficos
Autores principales: Cabernard, Clemens, Prehoda, Kenneth E., Doe, Chris Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028831/
https://www.ncbi.nlm.nih.gov/pubmed/20811457
http://dx.doi.org/10.1038/nature09334
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author Cabernard, Clemens
Prehoda, Kenneth E.
Doe, Chris Q.
author_facet Cabernard, Clemens
Prehoda, Kenneth E.
Doe, Chris Q.
author_sort Cabernard, Clemens
collection PubMed
description The mitotic spindle determines the cleavage furrow site during metazoan cell division(1,2), but whether other mechanisms exist remains unknown. Here we identify a spindle-independent mechanism for cleavage furrow positioning in Drosophila neuroblasts. We show that early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast basal cortex at anaphase onset by a Pins cortical polarity pathway, and can induce a basally-displaced furrow even in the complete absence of a mitotic spindle. Rotation or displacement of the spindle results in two furrows: an early polarity-induced basal furrow and a later spindle-induced furrow. This spindle-independent cleavage furrow mechanism may be relevant to other highly polarized mitotic cells, such as mammalian neural progenitors.
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spelling pubmed-40288312014-05-21 A mitotic spindle-independent cleavage furrow positioning pathway Cabernard, Clemens Prehoda, Kenneth E. Doe, Chris Q. Nature Article The mitotic spindle determines the cleavage furrow site during metazoan cell division(1,2), but whether other mechanisms exist remains unknown. Here we identify a spindle-independent mechanism for cleavage furrow positioning in Drosophila neuroblasts. We show that early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast basal cortex at anaphase onset by a Pins cortical polarity pathway, and can induce a basally-displaced furrow even in the complete absence of a mitotic spindle. Rotation or displacement of the spindle results in two furrows: an early polarity-induced basal furrow and a later spindle-induced furrow. This spindle-independent cleavage furrow mechanism may be relevant to other highly polarized mitotic cells, such as mammalian neural progenitors. 2010-09-02 /pmc/articles/PMC4028831/ /pubmed/20811457 http://dx.doi.org/10.1038/nature09334 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Cabernard, Clemens
Prehoda, Kenneth E.
Doe, Chris Q.
A mitotic spindle-independent cleavage furrow positioning pathway
title A mitotic spindle-independent cleavage furrow positioning pathway
title_full A mitotic spindle-independent cleavage furrow positioning pathway
title_fullStr A mitotic spindle-independent cleavage furrow positioning pathway
title_full_unstemmed A mitotic spindle-independent cleavage furrow positioning pathway
title_short A mitotic spindle-independent cleavage furrow positioning pathway
title_sort mitotic spindle-independent cleavage furrow positioning pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028831/
https://www.ncbi.nlm.nih.gov/pubmed/20811457
http://dx.doi.org/10.1038/nature09334
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