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Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa

Honey has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity a...

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Autores principales: Hayashi, Katsuhiko, Fukushima, Aiko, Hayashi-Nishino, Mitsuko, Nishino, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029012/
https://www.ncbi.nlm.nih.gov/pubmed/24860556
http://dx.doi.org/10.3389/fmicb.2014.00180
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author Hayashi, Katsuhiko
Fukushima, Aiko
Hayashi-Nishino, Mitsuko
Nishino, Kunihiko
author_facet Hayashi, Katsuhiko
Fukushima, Aiko
Hayashi-Nishino, Mitsuko
Nishino, Kunihiko
author_sort Hayashi, Katsuhiko
collection PubMed
description Honey has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, there is not much information describing its activity against gram-negative bacteria. In this study, we report the effect of methylglyoxal against multidrug-resistant Pseudomonas aeruginosa (MDRP) using 53 clinically isolated strains. We also assessed the effect of deleting the five multidrug efflux systems in P. aeruginosa, as well as the efflux systems in Escherichia coli and Salmonella enterica serovar Typhimurium, on MICs of methylglyoxal. Our results indicate that methylglyoxal inhibits the growth of MDRP at concentrations of 128–512 μg/ml (1.7–7.1 mM) and is not recognized by drug efflux systems.
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spelling pubmed-40290122014-05-23 Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa Hayashi, Katsuhiko Fukushima, Aiko Hayashi-Nishino, Mitsuko Nishino, Kunihiko Front Microbiol Microbiology Honey has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, there is not much information describing its activity against gram-negative bacteria. In this study, we report the effect of methylglyoxal against multidrug-resistant Pseudomonas aeruginosa (MDRP) using 53 clinically isolated strains. We also assessed the effect of deleting the five multidrug efflux systems in P. aeruginosa, as well as the efflux systems in Escherichia coli and Salmonella enterica serovar Typhimurium, on MICs of methylglyoxal. Our results indicate that methylglyoxal inhibits the growth of MDRP at concentrations of 128–512 μg/ml (1.7–7.1 mM) and is not recognized by drug efflux systems. Frontiers Media S.A. 2014-04-17 /pmc/articles/PMC4029012/ /pubmed/24860556 http://dx.doi.org/10.3389/fmicb.2014.00180 Text en Copyright © 2014 Hayashi, Fukushima, Hayashi-Nishino and Nishino. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hayashi, Katsuhiko
Fukushima, Aiko
Hayashi-Nishino, Mitsuko
Nishino, Kunihiko
Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title_full Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title_fullStr Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title_full_unstemmed Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title_short Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
title_sort effect of methylglyoxal on multidrug-resistant pseudomonas aeruginosa
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029012/
https://www.ncbi.nlm.nih.gov/pubmed/24860556
http://dx.doi.org/10.3389/fmicb.2014.00180
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