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Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice

Molecular mechanisms which stabilize dendrites and dendritic spines are essential for regulation of neuronal plasticity in development and adulthood. The class of Nogo receptor proteins, which are critical for restricting neurite outgrowth inhibition signaling, have been shown to have roles in devel...

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Autores principales: Borrie, Sarah C., Sartori, Simone B., Lehmann, Julian, Sah, Anupam, Singewald, Nicolas, Bandtlow, Christine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030173/
https://www.ncbi.nlm.nih.gov/pubmed/24860456
http://dx.doi.org/10.3389/fnbeh.2014.00175
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author Borrie, Sarah C.
Sartori, Simone B.
Lehmann, Julian
Sah, Anupam
Singewald, Nicolas
Bandtlow, Christine E.
author_facet Borrie, Sarah C.
Sartori, Simone B.
Lehmann, Julian
Sah, Anupam
Singewald, Nicolas
Bandtlow, Christine E.
author_sort Borrie, Sarah C.
collection PubMed
description Molecular mechanisms which stabilize dendrites and dendritic spines are essential for regulation of neuronal plasticity in development and adulthood. The class of Nogo receptor proteins, which are critical for restricting neurite outgrowth inhibition signaling, have been shown to have roles in developmental, experience and activity induced plasticity. Here we investigated the role of the Nogo receptor homolog NgR2 in structural plasticity in a transgenic null mutant for NgR2. Using Golgi-Cox staining to analyze morphology, we show that loss of NgR2 alters spine morphology in adult CA1 pyramidal neurons of the hippocampus, significantly increasing mushroom-type spines, without altering dendritic tree complexity. Furthermore, this shift is specific to apical dendrites in distal CA1 stratum radiatum (SR). Behavioral alterations in NgR2(−/−) mice were investigated using a battery of standardized tests and showed that whilst there were no alterations in learning and memory in NgR2(−/−) mice compared to littermate controls, NgR2(−/−) displayed reduced fear expression in the contextual conditioned fear test, and exhibited reduced anxiety- and depression-related behaviors. This suggests that the loss of NgR2 results in a specific phenotype of reduced emotionality. We conclude that NgR2 has role in maintenance of mature spines and may also regulate fear and anxiety-like behaviors.
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spelling pubmed-40301732014-05-23 Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice Borrie, Sarah C. Sartori, Simone B. Lehmann, Julian Sah, Anupam Singewald, Nicolas Bandtlow, Christine E. Front Behav Neurosci Neuroscience Molecular mechanisms which stabilize dendrites and dendritic spines are essential for regulation of neuronal plasticity in development and adulthood. The class of Nogo receptor proteins, which are critical for restricting neurite outgrowth inhibition signaling, have been shown to have roles in developmental, experience and activity induced plasticity. Here we investigated the role of the Nogo receptor homolog NgR2 in structural plasticity in a transgenic null mutant for NgR2. Using Golgi-Cox staining to analyze morphology, we show that loss of NgR2 alters spine morphology in adult CA1 pyramidal neurons of the hippocampus, significantly increasing mushroom-type spines, without altering dendritic tree complexity. Furthermore, this shift is specific to apical dendrites in distal CA1 stratum radiatum (SR). Behavioral alterations in NgR2(−/−) mice were investigated using a battery of standardized tests and showed that whilst there were no alterations in learning and memory in NgR2(−/−) mice compared to littermate controls, NgR2(−/−) displayed reduced fear expression in the contextual conditioned fear test, and exhibited reduced anxiety- and depression-related behaviors. This suggests that the loss of NgR2 results in a specific phenotype of reduced emotionality. We conclude that NgR2 has role in maintenance of mature spines and may also regulate fear and anxiety-like behaviors. Frontiers Media S.A. 2014-05-15 /pmc/articles/PMC4030173/ /pubmed/24860456 http://dx.doi.org/10.3389/fnbeh.2014.00175 Text en Copyright © 2014 Borrie, Sartori, Lehmann, Sah, Singewald and Bandtlow. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Borrie, Sarah C.
Sartori, Simone B.
Lehmann, Julian
Sah, Anupam
Singewald, Nicolas
Bandtlow, Christine E.
Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title_full Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title_fullStr Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title_full_unstemmed Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title_short Loss of Nogo receptor homolog NgR2 alters spine morphology of CA1 neurons and emotionality in adult mice
title_sort loss of nogo receptor homolog ngr2 alters spine morphology of ca1 neurons and emotionality in adult mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030173/
https://www.ncbi.nlm.nih.gov/pubmed/24860456
http://dx.doi.org/10.3389/fnbeh.2014.00175
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