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Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

BACKGROUND: Global climate change will noticeably affect plant vegetative and reproductive development. The recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. Physiological processes underlying temperature response and tolerance...

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Autores principales: Rienth, Markus, Torregrosa, Laurent, Luchaire, Nathalie, Chatbanyong, Ratthaphon, Lecourieux, David, Kelly, Mary T, Romieu, Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030582/
https://www.ncbi.nlm.nih.gov/pubmed/24774299
http://dx.doi.org/10.1186/1471-2229-14-108
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author Rienth, Markus
Torregrosa, Laurent
Luchaire, Nathalie
Chatbanyong, Ratthaphon
Lecourieux, David
Kelly, Mary T
Romieu, Charles
author_facet Rienth, Markus
Torregrosa, Laurent
Luchaire, Nathalie
Chatbanyong, Ratthaphon
Lecourieux, David
Kelly, Mary T
Romieu, Charles
author_sort Rienth, Markus
collection PubMed
description BACKGROUND: Global climate change will noticeably affect plant vegetative and reproductive development. The recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. Physiological processes underlying temperature response and tolerance of the grapevine fruit have not been extensively investigated. To date, all studies investigating the molecular regulation of fleshly fruit response to abiotic stress were only conducted during the day, overlooking possible critical night-specific variations. The present study explores the night and day transcriptomic response of grapevine fruit to heat stress at several developmental stages. Short heat stresses (2 h) were applied at day and night to vines bearing clusters sequentially ordered according to the developmental stages along their vertical axes. The recently proposed microvine model (DRCF-Dwarf Rapid Cycling and Continuous Flowering) was grown in climatic chambers in order to circumvent common constraints and biases inevitable in field experiments with perennial macrovines. Post-véraison berry heterogeneity within clusters was avoided by constituting homogenous batches following organic acids and sugars measurements of individual berries. A whole genome transcriptomic approach was subsequently conducted using NimbleGen 090818 Vitis 12X (30 K) microarrays. RESULTS: Present work reveals significant differences in heat stress responsive pathways according to day or night treatment, in particular regarding genes associated with acidity and phenylpropanoid metabolism. Precise distinction of ripening stages led to stage-specific detection of malic acid and anthocyanin-related transcripts modulated by heat stress. Important changes in cell wall modification related processes as well as indications for heat-induced delay of ripening and sugar accumulation were observed at véraison, an effect that was reversed at later stages. CONCLUSIONS: This first day - night study on heat stress adaption of the grapevine berry shows that the transcriptome of fleshy fruits is differentially affected by abiotic stress at night. The present results emphasize the necessity of including different developmental stages and especially several daytime points in transcriptomic studies.
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spelling pubmed-40305822014-05-23 Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit Rienth, Markus Torregrosa, Laurent Luchaire, Nathalie Chatbanyong, Ratthaphon Lecourieux, David Kelly, Mary T Romieu, Charles BMC Plant Biol Research Article BACKGROUND: Global climate change will noticeably affect plant vegetative and reproductive development. The recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. Physiological processes underlying temperature response and tolerance of the grapevine fruit have not been extensively investigated. To date, all studies investigating the molecular regulation of fleshly fruit response to abiotic stress were only conducted during the day, overlooking possible critical night-specific variations. The present study explores the night and day transcriptomic response of grapevine fruit to heat stress at several developmental stages. Short heat stresses (2 h) were applied at day and night to vines bearing clusters sequentially ordered according to the developmental stages along their vertical axes. The recently proposed microvine model (DRCF-Dwarf Rapid Cycling and Continuous Flowering) was grown in climatic chambers in order to circumvent common constraints and biases inevitable in field experiments with perennial macrovines. Post-véraison berry heterogeneity within clusters was avoided by constituting homogenous batches following organic acids and sugars measurements of individual berries. A whole genome transcriptomic approach was subsequently conducted using NimbleGen 090818 Vitis 12X (30 K) microarrays. RESULTS: Present work reveals significant differences in heat stress responsive pathways according to day or night treatment, in particular regarding genes associated with acidity and phenylpropanoid metabolism. Precise distinction of ripening stages led to stage-specific detection of malic acid and anthocyanin-related transcripts modulated by heat stress. Important changes in cell wall modification related processes as well as indications for heat-induced delay of ripening and sugar accumulation were observed at véraison, an effect that was reversed at later stages. CONCLUSIONS: This first day - night study on heat stress adaption of the grapevine berry shows that the transcriptome of fleshy fruits is differentially affected by abiotic stress at night. The present results emphasize the necessity of including different developmental stages and especially several daytime points in transcriptomic studies. BioMed Central 2014-04-28 /pmc/articles/PMC4030582/ /pubmed/24774299 http://dx.doi.org/10.1186/1471-2229-14-108 Text en Copyright © 2014 Rienth et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rienth, Markus
Torregrosa, Laurent
Luchaire, Nathalie
Chatbanyong, Ratthaphon
Lecourieux, David
Kelly, Mary T
Romieu, Charles
Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title_full Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title_fullStr Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title_full_unstemmed Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title_short Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
title_sort day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030582/
https://www.ncbi.nlm.nih.gov/pubmed/24774299
http://dx.doi.org/10.1186/1471-2229-14-108
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