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Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes

A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collag...

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Autores principales: Koh, Li Buay, Islam, Mohammad Mirazul, Mitra, Debbie, Noel, Christopher W., Merrett, Kimberley, Odorcic, Silvia, Fagerholm, Per, Jackson, William. Bruce, Liedberg, Bo, Phopase, Jaywant, Griffith, May
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030907/
https://www.ncbi.nlm.nih.gov/pubmed/24956085
http://dx.doi.org/10.3390/jfb4030162
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author Koh, Li Buay
Islam, Mohammad Mirazul
Mitra, Debbie
Noel, Christopher W.
Merrett, Kimberley
Odorcic, Silvia
Fagerholm, Per
Jackson, William. Bruce
Liedberg, Bo
Phopase, Jaywant
Griffith, May
author_facet Koh, Li Buay
Islam, Mohammad Mirazul
Mitra, Debbie
Noel, Christopher W.
Merrett, Kimberley
Odorcic, Silvia
Fagerholm, Per
Jackson, William. Bruce
Liedberg, Bo
Phopase, Jaywant
Griffith, May
author_sort Koh, Li Buay
collection PubMed
description A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF(4))(2)·XH(2)O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers.
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spelling pubmed-40309072014-06-12 Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes Koh, Li Buay Islam, Mohammad Mirazul Mitra, Debbie Noel, Christopher W. Merrett, Kimberley Odorcic, Silvia Fagerholm, Per Jackson, William. Bruce Liedberg, Bo Phopase, Jaywant Griffith, May J Funct Biomater Article A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF(4))(2)·XH(2)O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers. MDPI 2013-08-28 /pmc/articles/PMC4030907/ /pubmed/24956085 http://dx.doi.org/10.3390/jfb4030162 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Koh, Li Buay
Islam, Mohammad Mirazul
Mitra, Debbie
Noel, Christopher W.
Merrett, Kimberley
Odorcic, Silvia
Fagerholm, Per
Jackson, William. Bruce
Liedberg, Bo
Phopase, Jaywant
Griffith, May
Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title_full Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title_fullStr Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title_full_unstemmed Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title_short Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
title_sort epoxy cross-linked collagen and collagen-laminin peptide hydrogels as corneal substitutes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030907/
https://www.ncbi.nlm.nih.gov/pubmed/24956085
http://dx.doi.org/10.3390/jfb4030162
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