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Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions
Charge-pair interactions between acidic and basic residues on the surface of collagen can promote stability as well as control specificity of molecular recognition. Heterotrimeric collagen peptides have been engineered de novo using either rational or computational methods, which in both cases optim...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030969/ https://www.ncbi.nlm.nih.gov/pubmed/24970200 http://dx.doi.org/10.3390/biom3040986 |
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author | Parmar, Avanish S. Joshi, Mihir Nosker, Patrick L. Hasan, Nida F. Nanda, Vikas |
author_facet | Parmar, Avanish S. Joshi, Mihir Nosker, Patrick L. Hasan, Nida F. Nanda, Vikas |
author_sort | Parmar, Avanish S. |
collection | PubMed |
description | Charge-pair interactions between acidic and basic residues on the surface of collagen can promote stability as well as control specificity of molecular recognition. Heterotrimeric collagen peptides have been engineered de novo using either rational or computational methods, which in both cases optimize networks of favorable charge-pair interactions in the target structure. Less understood is the role of electrostatic repulsion between groups of like charge in destabilizing structure or directing molecular recognition. To study this, we apply a “charge crowding” approach, where repulsive interactions between multiple aspartate side chains are found to destabilize the homotrimer states in triple helical peptide system and can be utilized to promote the formation of heterotrimers. Neutralizing surface charge by increasing salt concentration or decreasing pH can enhance homotrimer stability, confirming the role of charge crowding on the destabilization of homotrimers via electrostatic repulsion. Charge crowding may be used in conjunction with other approaches to create specific collagen heterotrimers. |
format | Online Article Text |
id | pubmed-4030969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-40309692014-06-24 Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions Parmar, Avanish S. Joshi, Mihir Nosker, Patrick L. Hasan, Nida F. Nanda, Vikas Biomolecules Article Charge-pair interactions between acidic and basic residues on the surface of collagen can promote stability as well as control specificity of molecular recognition. Heterotrimeric collagen peptides have been engineered de novo using either rational or computational methods, which in both cases optimize networks of favorable charge-pair interactions in the target structure. Less understood is the role of electrostatic repulsion between groups of like charge in destabilizing structure or directing molecular recognition. To study this, we apply a “charge crowding” approach, where repulsive interactions between multiple aspartate side chains are found to destabilize the homotrimer states in triple helical peptide system and can be utilized to promote the formation of heterotrimers. Neutralizing surface charge by increasing salt concentration or decreasing pH can enhance homotrimer stability, confirming the role of charge crowding on the destabilization of homotrimers via electrostatic repulsion. Charge crowding may be used in conjunction with other approaches to create specific collagen heterotrimers. MDPI 2013-12-05 /pmc/articles/PMC4030969/ /pubmed/24970200 http://dx.doi.org/10.3390/biom3040986 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Parmar, Avanish S. Joshi, Mihir Nosker, Patrick L. Hasan, Nida F. Nanda, Vikas Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title | Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title_full | Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title_fullStr | Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title_full_unstemmed | Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title_short | Control of Collagen Stability and Heterotrimer Specificity through Repulsive Electrostatic Interactions |
title_sort | control of collagen stability and heterotrimer specificity through repulsive electrostatic interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030969/ https://www.ncbi.nlm.nih.gov/pubmed/24970200 http://dx.doi.org/10.3390/biom3040986 |
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