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A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization

Heart valve disease (HVD) is a globally increasing problem and accounts for thousands of deaths yearly. Currently end-stage HVD can only be treated by total valve replacement, however with major drawbacks. To overcome the limitations of conventional substitutes, a new clinical approach based on cell...

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Autores principales: König, Fabian, Hollweck, Trixi, Pfeifer, Stefan, Reichart, Bruno, Wintermantel, Erich, Hagl, Christian, Akra, Bassil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031004/
https://www.ncbi.nlm.nih.gov/pubmed/24955628
http://dx.doi.org/10.3390/jfb3030480
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author König, Fabian
Hollweck, Trixi
Pfeifer, Stefan
Reichart, Bruno
Wintermantel, Erich
Hagl, Christian
Akra, Bassil
author_facet König, Fabian
Hollweck, Trixi
Pfeifer, Stefan
Reichart, Bruno
Wintermantel, Erich
Hagl, Christian
Akra, Bassil
author_sort König, Fabian
collection PubMed
description Heart valve disease (HVD) is a globally increasing problem and accounts for thousands of deaths yearly. Currently end-stage HVD can only be treated by total valve replacement, however with major drawbacks. To overcome the limitations of conventional substitutes, a new clinical approach based on cell colonization of artificially manufactured heart valves has been developed. Even though this attempt seems promising, a confluent and stable cell layer has not yet been achieved due to the high stresses present in this area of the human heart. This study describes a bioreactor with a new approach to cell conditioning of tissue engineered heart valves. The bioreactor provides a low pulsatile flow that grants the correct opening and closing of the valve without high shear stresses. The flow rate can be regulated allowing a steady and sensitive conditioning process. Furthermore, the correct functioning of the valve can be monitored by endoscope surveillance in real-time. The tubeless and modular design allows an accurate, simple and faultless assembly of the reactor in a laminar flow chamber. It can be concluded that the bioreactor provides a strong tool for dynamic pre-conditioning and monitoring of colonized heart valve prostheses physiologically exposed to shear stress.
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spelling pubmed-40310042014-06-12 A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization König, Fabian Hollweck, Trixi Pfeifer, Stefan Reichart, Bruno Wintermantel, Erich Hagl, Christian Akra, Bassil J Funct Biomater Article Heart valve disease (HVD) is a globally increasing problem and accounts for thousands of deaths yearly. Currently end-stage HVD can only be treated by total valve replacement, however with major drawbacks. To overcome the limitations of conventional substitutes, a new clinical approach based on cell colonization of artificially manufactured heart valves has been developed. Even though this attempt seems promising, a confluent and stable cell layer has not yet been achieved due to the high stresses present in this area of the human heart. This study describes a bioreactor with a new approach to cell conditioning of tissue engineered heart valves. The bioreactor provides a low pulsatile flow that grants the correct opening and closing of the valve without high shear stresses. The flow rate can be regulated allowing a steady and sensitive conditioning process. Furthermore, the correct functioning of the valve can be monitored by endoscope surveillance in real-time. The tubeless and modular design allows an accurate, simple and faultless assembly of the reactor in a laminar flow chamber. It can be concluded that the bioreactor provides a strong tool for dynamic pre-conditioning and monitoring of colonized heart valve prostheses physiologically exposed to shear stress. MDPI 2012-07-19 /pmc/articles/PMC4031004/ /pubmed/24955628 http://dx.doi.org/10.3390/jfb3030480 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
König, Fabian
Hollweck, Trixi
Pfeifer, Stefan
Reichart, Bruno
Wintermantel, Erich
Hagl, Christian
Akra, Bassil
A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title_full A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title_fullStr A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title_full_unstemmed A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title_short A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization
title_sort pulsatile bioreactor for conditioning of tissue-engineered cardiovascular constructs under endoscopic visualization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031004/
https://www.ncbi.nlm.nih.gov/pubmed/24955628
http://dx.doi.org/10.3390/jfb3030480
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