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Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers

A recombinant 130 kDa dihemoglobin which is made up of a single-chain tetra-α globin and four β globins has been expressed as a soluble protein in E. coli. The sequence of the single chain tetra-α is: αI-Gly-αII-(SerGlyGly)5Ser-αIII-Gly-αIV. This dihemoglobin has been purified and characterized in v...

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Detalles Bibliográficos
Autores principales:	Marquardt, David A., Doyle, Michael P., Davidson, Jeffrey S., Epp, Janet K., Aitken, Jacqueline F., Lemon, Douglas D., Anthony-Cahill, Spencer J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031019/ https://www.ncbi.nlm.nih.gov/pubmed/24956516 http://dx.doi.org/10.3390/jfb3010061

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