Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples

A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extra...

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Autores principales: Roy, Béatrice, Duy, Sung Vo, Puy, Jean-Yves, Martin, Charlotte, Guitton, Jérome, Dumontet, Charles, Périgaud, Christian, Lefebvre-Tournier, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031021/
https://www.ncbi.nlm.nih.gov/pubmed/24956520
http://dx.doi.org/10.3390/jfb3010131
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author Roy, Béatrice
Duy, Sung Vo
Puy, Jean-Yves
Martin, Charlotte
Guitton, Jérome
Dumontet, Charles
Périgaud, Christian
Lefebvre-Tournier, Isabelle
author_facet Roy, Béatrice
Duy, Sung Vo
Puy, Jean-Yves
Martin, Charlotte
Guitton, Jérome
Dumontet, Charles
Périgaud, Christian
Lefebvre-Tournier, Isabelle
author_sort Roy, Béatrice
collection PubMed
description A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extraction of irinotecan from various organic media. A specific capacity of 0.65 µmol·g(−1) for the MIP was determined. The high specificity of this MIP was demonstrated by studying the retention behaviour of two related compounds, camptothecin and SN-38. This support was applied for the extraction of irinotecan from human serum samples.
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spelling pubmed-40310212014-06-12 Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples Roy, Béatrice Duy, Sung Vo Puy, Jean-Yves Martin, Charlotte Guitton, Jérome Dumontet, Charles Périgaud, Christian Lefebvre-Tournier, Isabelle J Funct Biomater Article A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extraction of irinotecan from various organic media. A specific capacity of 0.65 µmol·g(−1) for the MIP was determined. The high specificity of this MIP was demonstrated by studying the retention behaviour of two related compounds, camptothecin and SN-38. This support was applied for the extraction of irinotecan from human serum samples. MDPI 2012-02-20 /pmc/articles/PMC4031021/ /pubmed/24956520 http://dx.doi.org/10.3390/jfb3010131 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Roy, Béatrice
Duy, Sung Vo
Puy, Jean-Yves
Martin, Charlotte
Guitton, Jérome
Dumontet, Charles
Périgaud, Christian
Lefebvre-Tournier, Isabelle
Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title_full Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title_fullStr Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title_full_unstemmed Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title_short Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples
title_sort synthesis and evaluation of a molecularly imprinted polymer for selective solid-phase extraction of irinotecan from human serum samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031021/
https://www.ncbi.nlm.nih.gov/pubmed/24956520
http://dx.doi.org/10.3390/jfb3010131
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