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Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding

Cistanches Herba (Rou Cong Rong), known as “Ginseng of the desert”, has a striking curative effect on strength and nourishment, especially in kidney reinforcement to strengthen yang. However, the two plant origins of Cistanches Herba, Cistanche deserticola and Cistanche tubulosa, vary in terms of ph...

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Detalles Bibliográficos
Autores principales: Zheng, Sihao, Jiang, Xue, Wu, Labin, Wang, Zenghui, Huang, Linfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031141/
https://www.ncbi.nlm.nih.gov/pubmed/24854031
http://dx.doi.org/10.1371/journal.pone.0098061
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author Zheng, Sihao
Jiang, Xue
Wu, Labin
Wang, Zenghui
Huang, Linfang
author_facet Zheng, Sihao
Jiang, Xue
Wu, Labin
Wang, Zenghui
Huang, Linfang
author_sort Zheng, Sihao
collection PubMed
description Cistanches Herba (Rou Cong Rong), known as “Ginseng of the desert”, has a striking curative effect on strength and nourishment, especially in kidney reinforcement to strengthen yang. However, the two plant origins of Cistanches Herba, Cistanche deserticola and Cistanche tubulosa, vary in terms of pharmacological action and chemical components. To discriminate the plant origin of Cistanches Herba, a combined method system of chemical and genetic –UPLC-QTOF/MS technology and DNA barcoding–were firstly employed in this study. The results indicated that three potential marker compounds (isomer of campneoside II, cistanoside C, and cistanoside A) were obtained to discriminate the two origins by PCA and OPLS-DA analyses. DNA barcoding enabled to differentiate two origins accurately. NJ tree showed that two origins clustered into two clades. Our findings demonstrate that the two origins of Cistanches Herba possess different chemical compositions and genetic variation. This is the first reported evaluation of two origins of Cistanches Herba, and the finding will facilitate quality control and its clinical application.
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spelling pubmed-40311412014-05-28 Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding Zheng, Sihao Jiang, Xue Wu, Labin Wang, Zenghui Huang, Linfang PLoS One Research Article Cistanches Herba (Rou Cong Rong), known as “Ginseng of the desert”, has a striking curative effect on strength and nourishment, especially in kidney reinforcement to strengthen yang. However, the two plant origins of Cistanches Herba, Cistanche deserticola and Cistanche tubulosa, vary in terms of pharmacological action and chemical components. To discriminate the plant origin of Cistanches Herba, a combined method system of chemical and genetic –UPLC-QTOF/MS technology and DNA barcoding–were firstly employed in this study. The results indicated that three potential marker compounds (isomer of campneoside II, cistanoside C, and cistanoside A) were obtained to discriminate the two origins by PCA and OPLS-DA analyses. DNA barcoding enabled to differentiate two origins accurately. NJ tree showed that two origins clustered into two clades. Our findings demonstrate that the two origins of Cistanches Herba possess different chemical compositions and genetic variation. This is the first reported evaluation of two origins of Cistanches Herba, and the finding will facilitate quality control and its clinical application. Public Library of Science 2014-05-22 /pmc/articles/PMC4031141/ /pubmed/24854031 http://dx.doi.org/10.1371/journal.pone.0098061 Text en © 2014 Zheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zheng, Sihao
Jiang, Xue
Wu, Labin
Wang, Zenghui
Huang, Linfang
Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title_full Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title_fullStr Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title_full_unstemmed Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title_short Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
title_sort chemical and genetic discrimination of cistanches herba based on uplc-qtof/ms and dna barcoding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031141/
https://www.ncbi.nlm.nih.gov/pubmed/24854031
http://dx.doi.org/10.1371/journal.pone.0098061
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