A manual method to obtain platelet rich plasma

OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP). METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and pla...

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Autores principales: Marques, Fabiana Paulino, Ingham, Sheila Jean McNeill, Forgas, Andrea, Franciozi, Carlos Eduardo da Silveira, Sasaki, Pedro Henrique, Abdalla, Rene Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Ortopedia e Traumatologia 2014
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031249/
https://www.ncbi.nlm.nih.gov/pubmed/24868183
http://dx.doi.org/10.1590/1413-78522014220200798
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author Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira
Sasaki, Pedro Henrique
Abdalla, Rene Jorge
author_facet Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira
Sasaki, Pedro Henrique
Abdalla, Rene Jorge
author_sort Marques, Fabiana Paulino
collection PubMed
description OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP). METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured. RESULTS: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times. CONCLUSION: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.
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spelling pubmed-40312492014-05-27 A manual method to obtain platelet rich plasma Marques, Fabiana Paulino Ingham, Sheila Jean McNeill Forgas, Andrea Franciozi, Carlos Eduardo da Silveira Sasaki, Pedro Henrique Abdalla, Rene Jorge Acta Ortop Bras Original Articles OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP). METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured. RESULTS: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times. CONCLUSION: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study. Sociedade Brasileira de Ortopedia e Traumatologia 2014 /pmc/articles/PMC4031249/ /pubmed/24868183 http://dx.doi.org/10.1590/1413-78522014220200798 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira
Sasaki, Pedro Henrique
Abdalla, Rene Jorge
A manual method to obtain platelet rich plasma
title A manual method to obtain platelet rich plasma
title_full A manual method to obtain platelet rich plasma
title_fullStr A manual method to obtain platelet rich plasma
title_full_unstemmed A manual method to obtain platelet rich plasma
title_short A manual method to obtain platelet rich plasma
title_sort manual method to obtain platelet rich plasma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031249/
https://www.ncbi.nlm.nih.gov/pubmed/24868183
http://dx.doi.org/10.1590/1413-78522014220200798
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